Linehan Jonathan L, Dileepan Thamotharampillai, Kashem Sakeen W, Kaplan Daniel H, Cleary Patrick, Jenkins Marc K
Department of Microbiology and Immunology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455;
Department of Dermatology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455.
Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12782-7. doi: 10.1073/pnas.1513532112. Epub 2015 Sep 28.
Intranasal (i.n.) infections preferentially generate Th17 cells. We explored the basis for this anatomic preference by tracking polyclonal CD4(+) T cells specific for an MHC class II-bound peptide from the mucosal pathogen Streptococcus pyogenes. S. pyogenes MHC class II-bound peptide-specific CD4(+) T cells were first activated in the cervical lymph nodes following i.n. inoculation and then differentiated into Th17 cells. S. pyogenes-induced Th17 formation depended on TGF-β1 from dendritic cells and IL-6 from a CD301b(+) dendritic cell subset located in the cervical lymph nodes but not the spleen. Thus, the tendency of i.n. infection to induce Th17 cells is related to cytokine production by specialized dendritic cells that drain this site.
鼻内（i.n.）感染优先产生Th17细胞。我们通过追踪针对黏膜病原体化脓性链球菌的MHC II类结合肽的多克隆CD4(+) T细胞，探索了这种解剖学偏好的基础。在鼻内接种后，化脓性链球菌MHC II类结合肽特异性CD4(+) T细胞首先在颈淋巴结中被激活，然后分化为Th17细胞。化脓性链球菌诱导的Th17细胞形成依赖于树突状细胞产生的TGF-β1和位于颈淋巴结而非脾脏中的CD301b(+)树突状细胞亚群产生的IL-6。因此，鼻内感染诱导Th17细胞的倾向与引流该部位的特殊树突状细胞产生的细胞因子有关。
