Izbicki J R, Ziegler-Heitbrock H W, Meier M, Lütticken R, Wilker D K, Siebeck M, Scheuber H P, Schweiberer L
Dept. of Surgery, University of Munich, FRG.
J Clin Lab Immunol. 1989 Sep;30(1):13-9.
Some controversy exists regarding the antibody response after splenectomy and spleen preserving operations. In a porcine model the specific IgG antibody response to tetanus toxoid and type 6B pneumococcal polysaccharide was studied in 10 animals with splenectomy, 11 animals with splenic resection, 10 animals with splenic autotransplantation and 10 sham operated control animals. The operative groups were divided in two subgroups, receiving either the immuno adjuvant MTP-PE or the vehicle alone. Specific antibodies were determined by ELISA. Immunization with tetanus toxoid led to slightly lower peak IgG levels in splenectomized animals, but this was statistically not significant as compared to controls. In addition, the distribution of responders (78%) was not influenced by type of operation. Type 6B-pneumococcal polysaccharide proved to be a weak immunogen (19% responders). Splenectomy or spleen preserving surgery had no impact on the proportion of responders and peak IgG antibody titers of responders to this antigen. Additional administration of MTP-PE did not significantly increase the proportion of responders and had no impact on peak IgG antibody levels to tetanus toxoid and type 6B pneumococcal polysaccharide. These results show in contrast to previous studies in man, that under controlled conditions in the porcine model serum antibody responses to T-cell dependent and T-cell independent antigens are only slightly decreased by splenectomy. In addition, no effects of spleen preserving operations on antibody response are observed, and there is no change after concomitant administration of a muramyl peptide.
关于脾切除术后和保留脾脏手术之后的抗体反应存在一些争议。在一个猪模型中，对10只接受脾切除术的动物、11只接受脾脏部分切除术的动物、10只接受脾脏自体移植的动物以及10只接受假手术的对照动物，研究了它们对破伤风类毒素和6B型肺炎球菌多糖的特异性IgG抗体反应。手术组被分为两个亚组，分别接受免疫佐剂MTP - PE或仅接受赋形剂。通过ELISA测定特异性抗体。用破伤风类毒素免疫后，脾切除动物的IgG峰值水平略低，但与对照组相比，这在统计学上无显著差异。此外，反应者的分布比例（78%）不受手术类型的影响。6B型肺炎球菌多糖被证明是一种弱免疫原（19%的反应者）。脾切除术或保留脾脏的手术对该抗原反应者的比例和反应者的IgG抗体峰值滴度没有影响。额外给予MTP - PE并没有显著增加反应者的比例，并且对破伤风类毒素和6B型肺炎球菌多糖的IgG抗体峰值水平没有影响。这些结果与之前对人类的研究形成对比，即在猪模型的受控条件下，脾切除术仅使对T细胞依赖性和T细胞非依赖性抗原的血清抗体反应略有下降。此外，未观察到保留脾脏手术对抗体反应有影响，并且在同时给予胞壁酰肽后也没有变化。
