Chen W, Ackerman J J
Department of Chemistry, Washington University, St. Louis, Missouri 63130-4899.
NMR Biomed. 1989 Dec;2(5-6):267-73. doi: 10.1002/nbm.1940020515.
An NMR spectroscopy localization method that employs an inhomogeneous surface-spoiling magnetic field gradient has been applied to detect the 13C-[1H] signals from deep-lying liver tissue of rat in vivo. The method requires small gradient driving currents (less than 1 amp) and short gradient periods (approximately 0.5-1.6 ms). Thus, it is not likely to suffer from induced eddy currents and can detect short T2 resonances. We demonstrate that by employing a pulsed surface-spoiling gradient one can eliminate "contaminating" signals from the surface-lying tissues (muscle, fat and skin) while still maintaining the resonance linewidth resolution for the deep-lying tissue of interest (liver). The feasibility of using this localization approach to trace glucose conversion into glycogen is shown.
一种采用非均匀表面扰相磁场梯度的核磁共振光谱定位方法已被应用于在体检测大鼠深部肝脏组织的13C-[1H]信号。该方法所需的梯度驱动电流较小（小于1安培），梯度周期较短（约0.5 - 1.6毫秒）。因此，它不太可能受到感应涡流的影响，并且能够检测短T2共振。我们证明，通过采用脉冲表面扰相梯度，可以消除来自表层组织（肌肉、脂肪和皮肤）的“污染”信号，同时仍能保持感兴趣的深部组织（肝脏）的共振线宽分辨率。展示了使用这种定位方法追踪葡萄糖转化为糖原的可行性。
