Du Liyu, Arnholt Kelly, Ripp Steven, Sayler Gary, Wang Siqun, Liang Chenghua, Wang Jingkuan, Zhuang Jie
College of Land and Environment, Shenyang Agriculture University, Shenyang, 110866, China.
The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, TN, 37996, USA.
Ecotoxicology. 2015 Dec;24(10):2049-53. doi: 10.1007/s10646-015-1555-0. Epub 2015 Sep 29.
The aim of this study was to evaluate the biological toxicity of cellulose nanocrystals (CNCs) using the constitutively bioluminescent luxCDABE-based bioreporter Escherichia coli 652T7. The effects of CNCs on E. c oli 652T7 biotoxicity were investigated at different CNC concentrations, reaction times, and IC50 values. CNC toxicity was also compared with and without ultrasonic dispersion to establish dispersibility effects. The results demonstrated that CNCs were not significantly toxic at concentrations at or below 250 mg/L. At concentrations higher than 300 mg/L, toxicity increased linearly as CNC concentrations increased up to 2000 mg/L. IC50 calculations demonstrated an increase in cytotoxicity as CNC exposure times increased, and elevated dispersibility of the CNCs were shown to increase cytotoxicity effects. These results suggest that CNCs can impact microbial populations if elevated concentration thresholds are met.
本研究的目的是使用基于组成型生物发光luxCDABE的生物报告菌株大肠杆菌652T7评估纤维素纳米晶体（CNC）的生物毒性。在不同的CNC浓度、反应时间和半数抑制浓度（IC50）值下，研究了CNC对大肠杆菌652T7生物毒性的影响。还比较了有无超声分散时CNC的毒性，以确定分散性的影响。结果表明，浓度在250 mg/L及以下时，CNC没有明显毒性。浓度高于300 mg/L时，随着CNC浓度增加至2000 mg/L，毒性呈线性增加。IC50计算表明，随着CNC暴露时间增加，细胞毒性增加，并且CNC分散性的提高显示出细胞毒性作用增强。这些结果表明，如果达到升高的浓度阈值，CNC会对微生物种群产生影响。
