Yan Shuai, Yue Yin-zi, Zeng Li, Yue Jin, Li Wen-lin, Mao Chun-qin, Yang Lan
Suzhou Hospital of Traditional Chinese Medicine, Suzhou, China.
Library of Nanjing University of Chinese Medicine, Nanjing, China.
J Obstet Gynaecol Res. 2015 Dec;41(12):1942-50. doi: 10.1111/jog.12807. Epub 2015 Sep 30.
We aimed to evaluate the preventive effect of the ligustrazine nanoparticles nano spray (LNNS) for postoperative peritoneal adhesions in female rat models.
Fifty Wistar female rats weighting 250-300 g were randomly assigned to seven equal groups. All animals in the seven groups underwent midline laparotomy and ceca were abraded with sterile rasp. Group 1 underwent sham operations without treatment. In group 2, a postoperative peritoneal adhesion model was created, but no medication was given. In group 3, a postoperative peritoneal adhesion model was treated with LNNS, 2.5 mg/kg. In group 4, a postoperative peritoneal adhesion model was treated with LNNS, 5 mg/kg. In group 5, a postoperative peritoneal adhesion model was treated with LNNS, 10 mg/kg. In group 6, a postoperative peritoneal adhesion model was treated with polylactic acid (PLA) nanoparticle. In group 7, a postoperative peritoneal adhesion model was treated with ligustrazine, 2.5 mg/kg. Ten days after surgery, macroscopic and pathologic assessments were performed, and peritoneal fluid samples were collected in each group. The levels of tumor necrosis factor-α, tissue plasminogen activator and plasminogen activator inhibitor-1 in peritoneal fluid were determined by enzyme-linked immunosorbent assay.
The adhesion score and extent of groups 4 and 5 was lower than that of group 2 in macroscopic assessment (P < 0.05). A comparison of tumor necrosis factor-α and tissue plasminogen activator level in the peritoneal fluid also demonstrated significant differences among groups 2, 4 and 5 (P < 0.05). The levels of plasminogen activator inhibitor-1 in peritoneal fluid in the LNNS groups were decreased compared to group 1.
We suggest that LNNS could reduce peritoneal adhesion formation and it could be applied as a novel intervention for postoperative peritoneal adhesion.
我们旨在评估川芎嗪纳米颗粒纳米喷雾剂（LNNS）对雌性大鼠术后腹膜粘连的预防作用。
将50只体重250 - 300克的Wistar雌性大鼠随机分为七组，每组数量相等。七组所有动物均行正中剖腹术，用无菌锉擦伤盲肠。第1组进行假手术，不进行治疗。第2组建立术后腹膜粘连模型，但不给予药物治疗。第3组术后腹膜粘连模型用2.5毫克/千克的LNNS治疗。第4组术后腹膜粘连模型用5毫克/千克的LNNS治疗。第5组术后腹膜粘连模型用10毫克/千克的LNNS治疗。第6组术后腹膜粘连模型用聚乳酸（PLA）纳米颗粒治疗。第7组术后腹膜粘连模型用2.5毫克/千克的川芎嗪治疗。术后10天，进行宏观和病理评估，并收集每组的腹膜液样本。通过酶联免疫吸附测定法测定腹膜液中肿瘤坏死因子-α、组织型纤溶酶原激活剂和纤溶酶原激活剂抑制剂-1的水平。
在宏观评估中，第4组和第5组的粘连评分和范围低于第2组（P < 0.05）。腹膜液中肿瘤坏死因子-α和组织型纤溶酶原激活剂水平在第2组、第4组和第5组之间的比较也显示出显著差异（P < 0.05）。与第1组相比，LNNS组腹膜液中纤溶酶原激活剂抑制剂-1的水平降低。
我们认为LNNS可以减少腹膜粘连的形成，并且可以作为术后腹膜粘连的一种新型干预措施应用。
