Duvoisin R M, Deneris E S, Patrick J, Heinemann S
Molecular Neurobiology Laboratory, Salk Institute, San Diego, California 92138.
Neuron. 1989 Oct;3(4):487-96. doi: 10.1016/0896-6273(89)90207-9.
A new nicotinic acetylcholine receptor (nAChR) subunit, beta 4, was identified by screening a rat genomic library. In situ hybridization histochemistry revealed expression of the beta 4 gene in the medial habenula of adult rat brains. The primary structure of this subunit was deduced from a cDNA clone isolated from a PC12 cDNA library. Functional nAChRs were detected in Xenopus oocytes injected in pairwise combinations with in vitro synthesized RNAs encoding beta 4 and either the alpha 2, alpha 3, or alpha 4 subunit. Unlike the alpha 3 beta 2 receptor, the alpha 3 beta 4 receptor is not blocked by bungarotoxin 3.1, indicating that the beta subunit can affect the sensitivity of neuronal nAChRs to this toxin. These results extend the functional diversity of nicotinic receptors in the nervous system.
通过筛选大鼠基因组文库鉴定出一种新的烟碱型乙酰胆碱受体（nAChR）亚基β4。原位杂交组织化学显示β4基因在成年大鼠脑内侧缰核中表达。该亚基的一级结构由从PC12 cDNA文库分离的一个cDNA克隆推导得出。在与体外合成的编码β4以及α2、α3或α4亚基的RNA以两两组合方式注射的非洲爪蟾卵母细胞中检测到了功能性nAChRs。与α3β2受体不同，α3β4受体不被银环蛇毒素3.1阻断，这表明β亚基可影响神经元nAChRs对该毒素的敏感性。这些结果扩展了神经系统中烟碱型受体的功能多样性。
