Moss B L, Schuetze S M, Role L W
Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Neuron. 1989 Nov;3(5):597-607. doi: 10.1016/0896-6273(89)90270-5.
Measurement of acetylcholine (ACh)-induced currents indicates that the sensitivity of embryonic sympathetic neurons increases following innervation in vivo and in vitro. We have used single-channel recording to assess the contribution of changes in ACh receptor properties to this increase. Early in development (before synaptogenesis), we detect three classes of ACh-activated channels that differ in their conductance and kinetics. Molecular studies indicating a variety of neuronal receptor subunit clones suggest a similar diversity. Later in development (after innervation), changes in functional properties include increases in conductance and apparent mean open time, the addition of a new conductance class, as well as apparent clustering and segregation of channel types. These changes in channel function are compatible with the developmental increase in ACh sensitivity.
乙酰胆碱（ACh）诱导电流的测量表明，胚胎交感神经元的敏感性在体内和体外神经支配后都会增加。我们使用单通道记录来评估ACh受体特性变化对这种增加的贡献。在发育早期（突触形成之前），我们检测到三类ACh激活通道，它们的电导和动力学不同。分子研究表明多种神经元受体亚基克隆提示了类似的多样性。在发育后期（神经支配之后），功能特性的变化包括电导增加和明显的平均开放时间增加、新电导类别的添加，以及通道类型的明显聚集和分离。通道功能的这些变化与ACh敏感性的发育性增加是一致的。
