Terplan G, Steinmeyer S
Chair of Hygiene and Technology of Milk, University of Munich, F.R.G.
Int J Food Microbiol. 1989 Jun;8(3):277-80. doi: 10.1016/0168-1605(89)90027-5.
To investigate the pathogenicity of Listeria spp., chick embryos were infected by two methods. Very encouraging results were obtained with the inoculation of the chorioallantoic membrane (CAM) of 10 day old chick embryos: embryos inoculated with pathogenic strains (L. monocytogenes or L. ivanovii) died within 72 h, whereas those infected with apathogenic strains survived. This test could be a suitable method to replace the mouse pathogenicity test.
为研究李斯特菌属的致病性，采用两种方法感染鸡胚。用10日龄鸡胚的绒毛尿囊膜（CAM）接种获得了非常令人鼓舞的结果：接种致病菌株（单核细胞增生李斯特菌或伊氏李斯特菌）的胚胎在72小时内死亡，而感染无致病力菌株的胚胎存活下来。该试验可能是替代小鼠致病性试验的合适方法。
