Pourahmad Fazel, Pate Mateja, Ocepek Matjaž, Borroni Emanuele, Cabibbe Andrea M, Capitolo Eleonora, Cittaro Davide, Frizzera Eliana, Jenčič Vlasta, Mariottini Alessandro, Marumo Kenji, Vaggelli Guendalina, Cirillo Daniela M, Tortoli Enrico
School of Veterinary Medicine, Ilam University, Ilam, Iran.
National Veterinary Institute, University of Ljubljana, Ljubljana, Slovenia.
Int J Syst Evol Microbiol. 2015 Dec;65(12):4724-4729. doi: 10.1099/ijsem.0.000642. Epub 2015 Sep 28.
The name 'Mycobacterium angelicum' dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. This name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. The four strains presented 100 % 16S rRNA gene sequence similarity with Mycobacterium szulgai but clearly differed from M. szulgai for the milky white aspect of the colonies. The sequence similarity with the type strain of M. szulgai ranged, in eight additionally investigated genetic targets, from 78.9 to 94.3 %, an evident contrast with the close relatedness that emerged at the level of 16S rRNA gene. The average nucleotide identity between the genomes of M. szulgai DSM 44166T and strain 126/5/03T (type strain of the novel species) was 92.92 %, and supported the status of independent species. The confirmation of the name Mycobacterium angelicum sp. nov. is proposed, with strain 126/5/03T ( = CIP 109313T = DSM 45057T) as the type strain.
“天使分枝杆菌”这一名称可追溯到2003年，当时它被提议用于命名从淡水神仙鱼中分离出的一种生长缓慢的分枝杆菌。在此重新启用该名称，并基于包括原始菌株在内的4株菌株的多相特征描述提出了这个新物种。这4株菌株与苏尔加分枝杆菌的16S rRNA基因序列相似度为100%，但在菌落呈乳白色方面与苏尔加分枝杆菌明显不同。在另外研究的8个基因靶点中，与苏尔加分枝杆菌模式菌株的序列相似度在78.9%至94.3%之间，这与16S rRNA基因水平上显示的密切相关性形成明显对比。苏尔加分枝杆菌DSM 44166T与菌株126/5/03T（新物种的模式菌株）基因组之间的平均核苷酸同一性为92.92%，支持其独立物种的地位。提议确认“天使分枝杆菌”这一新名称，模式菌株为菌株126/5/03T（=CIP 109313T=DSM 45057T）。
