Tortoli Enrico, Richter Elvira, Borroni Emanuele, Cabibbe Andrea M, Capitolo Eleonora, Cittaro Davide, Engel Regina, Hendricks Oliver, Hillemann Doris, Kristiansen Jette E, Mariottini Alessandro, Schubert Sabine, Cirillo Daniela M
Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
TB-Laboratory, Labor Limbach, Heidelberg, Germany.
Int J Syst Evol Microbiol. 2016 Jan;66(1):450-456. doi: 10.1099/ijsem.0.000744. Epub 2015 Nov 5.
The name 'Mycobacterium alsiense', although reported in 2007, has not been validly published. Polyphasic characterization of three available strains of this species led us to the conclusion that they represent a distinct species within the genus Mycobacterium. The proposed novel species grows slowly and presents pale yellow-pigmented colonies. Differentiation from other mycobacteria is not feasible on the basis of biochemical and cultural features alone while genetic analysis, extended to eight housekeeping genes and one spacer region, reveals its clear distinction from all other mycobacteria. Mycobacterium asiaticum is the most closely related species on the basis of 16S rRNA gene sequences (similarity 99.3 %); the average nucleotide identity between the genomes of the two species is 80.72 %, clearly below the suggested cut-off (95-96 %). The name Mycobacterium alsense sp. nov. is proposed here for the novel species and replaces the name 'M. alsiense', ex Richter et al. 2007, given at the time of isolation of the first strain. The type strain is TB 1906T ( = DSM 45230T = CCUG 56586T).
“阿氏分枝杆菌”这一名称虽于2007年被报道，但尚未有效发表。对该物种现有三株菌株进行的多相特征分析使我们得出结论，它们代表分枝杆菌属内一个独特的物种。该提议的新物种生长缓慢，菌落呈淡黄色。仅根据生化和培养特征无法与其他分枝杆菌区分开来，而对八个管家基因和一个间隔区进行的遗传分析表明，它与所有其他分枝杆菌有明显区别。基于16S rRNA基因序列，亚洲分枝杆菌是最密切相关的物种（相似度99.3%）；两个物种基因组之间的平均核苷酸同一性为80.72%，明显低于建议的阈值（95 - 96%）。在此提议将该新物种命名为阿氏分枝杆菌新种（Mycobacterium alsense sp. nov.），取代里希特等人在2007年首次分离菌株时给出的名称“阿氏分枝杆菌（M. alsiense）”。模式菌株为TB 1906T（= DSM 45230T = CCUG 56586T）。
