Hashemi Shahraki Abdolrazagh, Trovato Alberto, Droz Sara, Haidarieh Parvin, Borroni Emanuele, Mirsaeidi Mehdi, Mannino Roberta, Hashemzadeh Mohamad, Mariottini Alessandro, Cirillo Daniela Maria, Tortoli Enrico
Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran.
Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3279-3282. doi: 10.1099/ijsem.0.002103. Epub 2017 Aug 22.
The characterization of five Iranian isolates, four from hospital haemodialysis water and one from the sputum of a patient, led to the detection of a novel mycobacterium species. The strains were characterized by mucoid colonies developing in 3-5 days at temperatures ranging from 25 to 37 °C. The biochemical test pattern was unremarkable while the HPLC profile of mycolic acids resembled that of Mycobacterium fortuitum. The sequences of three major housekeeping genes (16S rRNA, hsp65 and rpoB) were unique and differed from those of any other mycobacterium. Mycobacterium brisbanense, which is the species that shared the highest 16S rRNA gene sequence similarity (99.03 %), was distinct, as shown by the average nucleotide identity and by the genome to genome distance values (91.05 and 43.10 %, respectively). The strains are thus considered to represent a novel species of the genus Mycobacterium, for which the name Mycobacterium aquaticum sp. nov. is proposed. The type strain is RW6T (=DSM 104277T=CIP111198T).
对五株伊朗分离株进行鉴定，其中四株来自医院血液透析用水，一株来自患者痰液，结果检测到一种新的分枝杆菌物种。这些菌株的特征是在25至37°C的温度下，3至5天内形成黏液样菌落。生化测试模式无明显特征，而分枝菌酸的高效液相色谱图谱与偶然分枝杆菌相似。三个主要管家基因（16S rRNA、hsp65和rpoB）的序列独特，与其他任何分枝杆菌的序列不同。布里斯班分枝杆菌与该菌株的16S rRNA基因序列相似度最高（99.03%），但通过平均核苷酸同一性和全基因组距离值（分别为91.05%和43.10%）可以看出，二者是不同的。因此，这些菌株被认为代表了分枝杆菌属的一个新物种，为此提出了水生分枝杆菌新种的名称。模式菌株为RW6T（=DSM 104277T=CIP111198T）。
