Fornell Anna, Nilsson Johan, Jonsson Linus, Periyannan Rajeswari Prem Kumar, Joensson Haakan N, Tenje Maria
Dept. Biomedical Engineering, Lund University , Box 118, S-221 00, Lund, Sweden.
Div. of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of Technology, Box 1031, S-171 21 Solna, Sweden.
Anal Chem. 2015 Oct 20;87(20):10521-6. doi: 10.1021/acs.analchem.5b02746. Epub 2015 Oct 9.
In this paper, we utilize bulk acoustic waves to control the position of microparticles inside droplets in two-phase microfluidic systems and demonstrate a method to enrich the microparticles. In droplet microfluidics, different unit operations are combined and integrated on-chip to miniaturize complex biochemical assays. We present a droplet unit operation capable of controlling the position of microparticles during a trident shaped droplet split. An acoustic standing wave field is generated in the microchannel, and the acoustic forces direct the encapsulated microparticles to the center of the droplets. The method is generic, requires no labeling of the microparticles, and is operated in a noncontact fashion. It was possible to achieve 2+-fold enrichment of polystyrene beads (5 μm in diameter) in the center daughter droplet with an average recovery of 89% of the beads. Red blood cells were also successfully manipulated inside droplets. These results show the possibility to use acoustophoresis in two-phase systems to enrich microparticles and open up the possibility for new droplet-based assays that are not performed today.
在本文中，我们利用体声波来控制两相微流控系统中液滴内微粒的位置，并展示了一种富集微粒的方法。在液滴微流控中，不同的单元操作被组合并集成在芯片上，以实现复杂生化分析的小型化。我们提出了一种在三叉形液滴分裂过程中能够控制微粒位置的液滴单元操作。在微通道中产生一个声驻波场，声力将封装的微粒引导至液滴中心。该方法具有通用性，无需对微粒进行标记，并且以非接触方式操作。在中心子液滴中实现了直径为5μm的聚苯乙烯珠粒2倍以上的富集，珠粒的平均回收率为89%。红细胞也在液滴内成功地得到了操控。这些结果表明在两相系统中使用声泳富集微粒的可能性，并为目前尚未开展的新型基于液滴的分析开辟了可能性。
