Pereira Leonor, Martins-Lopes Paula
University of Trás-os-Montes and Alto Douro , P.O. Box 1013, 5000-911 Vila Real, Portugal.
Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute, University of Lisboa , Campo Grande, 1749-016 Lisboa, Portugal.
J Agric Food Chem. 2015 Oct 21;63(41):9165-74. doi: 10.1021/acs.jafc.5b03463. Epub 2015 Oct 8.
Vitis vinifera L. is a species with a large number of varieties, which differ in terms of anthocyanin content. The genes involved in the anthocyanin biosynthesis pathway have a direct effect in the anthocyanin profile of each variety, being potentially interesting for varietal identification. The current study aimed at the design of an assay suitable for the discrimination of the largest number of grapevine varieties. Two genes of the anthocyanin pathway, chalcone isomerase (CHI) and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT), were sequenced in 22 grapevine varieties. The CHI gene presented 5 SNPs within the sequence. A total of 58 SNPs and 1 INDEL were found among the UFGT gene, allowing the discrimination of 18 different genotypes within the 22 grapevine varieties. A HRM assay designed for UFGT, containing 704 bp, produced differentiated melting curves for each of the 18 haplotypes. The developed HRM assay is efficient in grapevine varietal discrimination.
欧亚葡萄（Vitis vinifera L.）是一个拥有大量品种的物种，这些品种在花青素含量方面存在差异。参与花青素生物合成途径的基因对每个品种的花青素谱有直接影响，这对于品种鉴定可能具有重要意义。当前的研究旨在设计一种适用于区分最多数量葡萄品种的检测方法。在22个葡萄品种中对花青素途径的两个基因，查尔酮异构酶（CHI）和UDP - 葡萄糖：类黄酮3 - O - 葡萄糖基转移酶（UFGT）进行了测序。CHI基因在序列中呈现出5个单核苷酸多态性（SNP）。在UFGT基因中总共发现了58个SNP和1个插入缺失（INDEL），能够在22个葡萄品种中区分出18种不同的基因型。针对UFGT设计的包含704 bp的高分辨率熔解曲线分析（HRM）检测方法，为18种单倍型中的每一种都产生了不同的熔解曲线。所开发的HRM检测方法在葡萄品种鉴别方面是有效的。
