Kjellman Ellen Eide, Slettemeås Jannice Schau, Small Harald, Sunde Marianne
Follo Dyreklinikk AS, 1400, Ski, Norway.
Norwegian Veterinary Institute, Pb 750 Sentrum, 0106, Oslo, Norway.
Microbiologyopen. 2015 Dec;4(6):857-66. doi: 10.1002/mbo3.258. Epub 2015 Oct 1.
The aim of the study was to investigate the occurrence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in healthy dogs and further to determine genetic relatedness between carrier isolates and clinical MRSP from dogs in Norway. A total of 189 healthy dogs visiting ten veterinary clinics were screened for MRSP during the period February to April 2013. Carrier isolates were susceptibility tested with disk diffusion and genotyped using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Forty-nine clinical MRSP were characterized for comparison. These isolates were collected from July 2008 to April 2013 and represent all MRSP index isolates from each MRSP-positive dog detected in Norway until April 2013. Geographical distribution of all MRSP cases was investigated using the ArcGIS 9.3 Software. MRSP was detected from five (2.6%) healthy dogs, sampled at three different clinics. The isolates grouped into three sequence types (STs): ST252 (two isolates), ST71 (two isolates) and ST306 (one isolate). MRSP from dogs sampled at the same animal clinic belonged to the same ST and produced identical PFGE pattern. The 49 clinical MRSP grouped into 15 STs; ST258 (n = 17), ST71 (n = 10), and ST305 (n = 4) were the most prevalent. The MRSP carrier isolates were genetically related to MRSP variants from dogs with infections as ST306 (from a carrier) is related to ST258. MRSP ST252, found in two carriers, was also present among the clinical MRSP isolates. Altogether the MRSP isolates were genetically diverse and MRSP of other lineages than ST71 continues to disseminate in Norway. Susceptibility testing showed that MRSP isolates of the ST71 lineage were the most multiresistant. Our study showed that MRSP could be detected in healthy dogs without infections and with no recent history of antimicrobial therapy stressing the need for future monitoring, infection control and prudent use of antimicrobial agents.
本研究的目的是调查健康犬中耐甲氧西林中间型葡萄球菌（MRSP）的发生情况，并进一步确定挪威犬的携带分离株与临床MRSP之间的遗传相关性。在2013年2月至4月期间，对前往十家兽医诊所就诊的189只健康犬进行了MRSP筛查。对携带分离株进行纸片扩散药敏试验，并使用多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）进行基因分型。对49株临床MRSP进行特征分析以作比较。这些分离株于2008年7月至2013年4月收集，代表了截至2013年4月在挪威检测到的每只MRSP阳性犬的所有MRSP索引分离株。使用ArcGIS 9.3软件调查了所有MRSP病例的地理分布。在三家不同诊所采集的五只（2.6%）健康犬中检测到MRSP。分离株分为三种序列类型（STs）：ST252（两株）、ST71（两株）和ST306（一株）。在同一家动物诊所采集的犬的MRSP属于同一ST，并产生相同的PFGE图谱。49株临床MRSP分为15种STs；最常见的是ST258（n = 17）、ST71（n = 10）和ST305（n = 4）。MRSP携带分离株与感染犬的MRSP变体存在遗传相关性，如ST306（来自携带者）与ST258相关。在两只携带者中发现的MRSP ST252也存在于临床MRSP分离株中。总体而言，MRSP分离株在遗传上具有多样性，除ST71外的其他谱系的MRSP在挪威仍在继续传播。药敏试验表明，ST71谱系的MRSP分离株耐药性最强。我们的研究表明，在无感染且近期无抗菌治疗史的健康犬中可检测到MRSP，这强调了未来监测、感染控制和谨慎使用抗菌药物的必要性。
