Suppr超能文献

韩国犬皮肤感染耐甲氧西林金黄色葡萄球菌分离株的克隆分布。

Clonal distribution of methicillin-resistant Staphylococcus pseudintermedius isolates from skin infection of dogs in Korea.

机构信息

Laboratory of Veterinary Dermatology, Republic of Korea.

Department of Companion Animal and Animal Resources Science, Joongbu University, Chungnam 32713, Republic of Korea.

出版信息

Vet Microbiol. 2017 Oct;210:32-37. doi: 10.1016/j.vetmic.2017.08.017. Epub 2017 Aug 30.

Abstract

Bacterial infection by methicillin-resistant Staphylococcus pseudintermedius (MRSP) is challenging in a small animal practice. Zoonotic transmission may occur. The aim of this study was to investigate the genotypic profiles of MRSP isolated from bacterial infections of canine skin in Korea and to compare their molecular lineages with dominant strains from other countries. Sixty MRSP isolates were obtained from the lesions of canine pyoderma and otitis externa. Their genetic diversity was assessed by multilocus sequence typing (MLST), staphylococcal protein A (spa) typing and direct-repeat unit (dru) typing. Staphylococcal cassette chromosome mec (SCCmec) elements were characterized by multiplex PCR. Thirty-nine different sequence types (STs) were detected. Among them, 21 STs were identified as internationally new sequence types. Fourteen dru types (dts) were detected, and the major types were dt11a and dt11y. spa typing characterised 21 isolates (35%, 21/60), including spa types t02 (n=8), t05 (n=5), t06 (n=6), and t15 (n=2). Two clonal complexes, CC568 and CC677, were revealed by MLST; this result differed from the dominant STs detected in MRSP isolates from Europe, North America, and other Asian countries. SCCmec type V was the major type (27/60. 45%), and 30 (50%) isolates were non-typeable by conventional classifying method. This is the first report about the clonal lineage of MRSP isolated from Korea. MRSP isolated from dogs in Korea displays independent lineage from other countries. Surveillance is needed to confirm cross-national disseminating patterns.

摘要

耐甲氧西林金黄色葡萄球菌(MRSP)引起的细菌感染在小动物临床实践中具有挑战性。可能会发生人畜共患传播。本研究旨在调查从韩国犬皮肤细菌感染中分离的 MRSP 的基因谱,并将其分子谱系与来自其他国家的优势菌株进行比较。从犬脓皮病和外耳炎的病变中获得了 60 株 MRSP 分离株。通过多位点序列分型(MLST)、葡萄球菌蛋白 A(spa)分型和直接重复单位(dru)分型评估其遗传多样性。通过多重 PCR 对葡萄球菌盒式染色体 mec(SCCmec)元件进行了特征分析。检测到 39 种不同的序列类型(ST)。其中,21 种 ST 被确定为国际新序列类型。检测到 14 种 dru 类型(dts),主要类型为 dt11a 和 dt11y。spa 分型鉴定了 21 株(35%,21/60),包括 spa 型 t02(n=8)、t05(n=5)、t06(n=6)和 t15(n=2)。通过 MLST 揭示了 2 个克隆复合体,CC568 和 CC677,这与从欧洲、北美和其他亚洲国家分离的 MRSP 分离株中检测到的优势 ST 不同。SCCmec 型 V 是主要类型(27/60. 45%),30 株(50%)通过传统分类方法无法定型。这是首次报道从韩国分离的 MRSP 的克隆谱系。韩国从犬中分离的 MRSP 与其他国家的分离株具有独立的谱系。需要进行监测以确认跨国传播模式。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验