Yeast, Saccharomyces cerevisiae, cell-free translation: the inhibition of translation by high temperature is reversible.

Yeast, Saccharomyces cerevisiae, extracts are inactive for translation at 37 degrees C. Two unexplained, simultaneously occurring phenomena appear to be responsible for this effect: (i) rapid inhibition of translation, and (ii) time-dependent inactivation of (a) translational component(s) at 37 degrees C. After short incubation of an extract at 37 degrees C, protein synthesis recovers efficiently after transfer of the extract to 23 degrees C. This behaviour of yeast cell-free systems enables the in vitro inactivation of temperature-sensitive translational components and therefore facilitates studies with extracts derived from temperature-sensitive strains.