Miller M J, Xuong N H, Geiduschek E P
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5222-5. doi: 10.1073/pnas.76.10.5222.
When Saccharomyces cerevisiae are subjected to a sudden increase in temperature (22 degrees C to 37 degrees C) they undergo extensive and, in some cases, extreme alterations in their rates of synthesizing individual polypeptides. These changes were monitored by pulse-labeling cells with [35S]methionine and separating the total soluble proteins by two-dimensional gel electrophoresis. Incorporation of 35S into individual proteins was measured by a computer-coupled autoradiogram-scanning method. The rates of synthesis of most proteins are transiently changed; 10-fold or greater induction or respression is common. This temperature response has also been studied in a mutant strain that is temperature sensitive for the nucleus-to-cytoplasm transport of RNA. In this mutant, not only the induction, but also a part of the repression in response to temperature upshift is largely inhibited. Conceivable mechanisms are discussed.
当酿酒酵母受到温度突然升高（从22摄氏度升至37摄氏度）的影响时，它们在合成单个多肽的速率上会发生广泛的，在某些情况下甚至是极端的变化。通过用[35S]甲硫氨酸脉冲标记细胞并用二维凝胶电泳分离总可溶性蛋白来监测这些变化。通过计算机耦合的放射自显影片扫描方法测量35S掺入单个蛋白质中的量。大多数蛋白质的合成速率会发生短暂变化；10倍或更大程度的诱导或抑制很常见。还在对RNA从细胞核到细胞质的转运对温度敏感的突变菌株中研究了这种温度反应。在这种突变体中，不仅诱导作用，而且响应温度升高的部分抑制作用也在很大程度上受到抑制。文中讨论了可能的机制。
