[呼吸道合胞病毒细支气管炎中炎症细胞因子和血浆皮质醇水平]
[Levels of inflammatory cytokines and plasma cortisol in respiratory syncytial virus bronchiolitis].
作者信息
Díaz Patricia V, Gaggero Aldo A, Pinto Ricardo A, Mamani Rossana, Uasapud Paola A, Bono María Rosa
机构信息
Programa Fisiopatología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
出版信息
Rev Med Chil. 2013 May;141(5):574-81. doi: 10.4067/S0034-98872013000500004.
BACKGROUND
An increased inflammatory innate response may play a role in pathogenesis of respiratory syncytial virus (RSV) infection.
AIM
To quantify pro-inflammatory cytokines (IL-6-IL-8, ÍL-2-P and TNF-a) in nasopharyngeal aspirate (NPA) and plasma, and plasma cortisol in previously healthy infants with RSV bronchiolitis.
PATIENTS AND METHODS
We studied 49 infants aged less than one year of age with RSV bronchiolitis and 25 healthy controls. Severity was defined using a previously described modified score. We quantified interleukins in NPA and plasma by flow cytometry and plasma cortisol by radioimmunoanalysis.
RESULTS
Among patients with RSV bronchiolitis, 25 were classified as severe and 24 as moderate or mild. Significantly higher levels of IL-6 and IL-8 in NPA and plasma and IL-lfi in NPA were found in children classified as severe, when compared to those with moderate or mild disease and controls. There was a positive correlation between IL-6 and cortisol in plasma (r = 0,55; p < 0,0001) and both were correlated with the severity of the disease.
CONCLUSIONS
RSV bronchiolitis severity was associated with higher levéis of inflammatory interleukins and plasma cortisol.
背景
炎症性固有免疫反应增强可能在呼吸道合胞病毒（RSV）感染的发病机制中起作用。
目的
对患有RSV细支气管炎的既往健康婴儿的鼻咽抽吸物（NPA）和血浆中的促炎细胞因子（IL-6 - IL-8、IL-2 - P和TNF-α）以及血浆皮质醇进行定量分析。
患者和方法
我们研究了49例年龄小于1岁的RSV细支气管炎婴儿和25例健康对照。使用先前描述的改良评分定义疾病严重程度。我们通过流式细胞术对NPA和血浆中的白细胞介素进行定量分析，并通过放射免疫分析对血浆皮质醇进行定量分析。
结果
在患有RSV细支气管炎的患者中，25例被分类为重度，24例为中度或轻度。与中度或轻度疾病患者及对照组相比，重度患者的NPA和血浆中IL-6和IL-8水平以及NPA中IL-1β水平显著更高。血浆中IL-6与皮质醇之间存在正相关（r = 0.55；p < 0.0001），且二者均与疾病严重程度相关。
结论
RSV细支气管炎的严重程度与炎症性白细胞介素和血浆皮质醇的较高水平相关。
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