Chen Ruohua, Zhou Xiang, Yu Zhenhai, Liu Jianjun, Huang Gang
From the Department of Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China (CRH, ZX, LJJ, HG); School of Biomedical Engineering, Shanghai Jiao Tong University (YZH); and Department of Cancer Metabolism, Institute of Health Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School Medicine, Shanghai, China (HG).
Medicine (Baltimore). 2015 Sep;94(39):e1583. doi: 10.1097/MD.0000000000001583.
Lactate dehydrogenase B (LDHB) is widely expressed in adult somatic tissue and plays important roles in the development of human cancers. However, the association between LDHB expression and the clinicopathological characteristics of hepatocellular carcinoma (HCC) is not well understood. The study was to detect the expression of LDHB in human HCC and investigate the association between its expression and the clinicopathological characteristics of HCC. Immunohistochemistry (IHC) analysis was performed to characterize the expression of LDHB in HCC and matched noncancerous tissues. Kaplan-Meier survival and Cox regression analyses were employed to evaluate the prognosis of 75 HCC patients. The results showed that the expression of LDHB in HCC was significantly lower than that in noncancerous tissues. Moreover, the expression level of the LDHB protein in HCC was correlated with pathological grade (P = 0.037), vascular invasion (P = 0.037), lymph node metastasis (P = 0.016), and tumor-node metastasis (TNM) stage (P = 0.007). Cox regression analysis further revealed that LDHB expression is an independent prognostic factor for disease-free survival (P = 0.045) and overall survival (P = 0.019).These data are the first to indicate that LDHB expression is a valuable prognostic biomarker for HCC and that low LDHB expression suggests unfavorable survival outcomes in HCC patients.
乳酸脱氢酶B（LDHB）在成人体组织中广泛表达，并在人类癌症发展中发挥重要作用。然而，LDHB表达与肝细胞癌（HCC）临床病理特征之间的关联尚未完全明确。本研究旨在检测LDHB在人类HCC中的表达，并探讨其表达与HCC临床病理特征之间的关联。采用免疫组织化学（IHC）分析来表征LDHB在HCC及配对的癌旁组织中的表达。运用Kaplan-Meier生存分析和Cox回归分析来评估75例HCC患者的预后。结果显示，LDHB在HCC中的表达显著低于癌旁组织。此外，HCC中LDHB蛋白的表达水平与病理分级（P = 0.037）、血管侵犯（P = 0.037）、淋巴结转移（P = 0.016）及肿瘤-淋巴结-转移（TNM）分期（P = 0.007）相关。Cox回归分析进一步显示，LDHB表达是无病生存期（P = 0.045）和总生存期（P = 0.019）的独立预后因素。这些数据首次表明，LDHB表达是HCC的一个有价值的预后生物标志物，且低LDHB表达提示HCC患者的生存结局不佳。
