Ganie Showkat Ahmad, Ali Dar Tanveer, Zargar Sabuhi, Bhat Aashiq Hussain, Dar Khalid Bashir, Masood Akbar, Zargar Mohammad Afzal
a Department of Clinical Biochemistry and.
b Department of Biochemistry , University of Kashmir , Srinagar , India.
Pharm Biol. 2016 Jul;54(7):1246-54. doi: 10.3109/13880209.2015.1066398. Epub 2015 Oct 1.
Crataegus songarica K. Koch (Rosaceae) has been used in folk medicine to treat various diseases.
This study evaluates the effect of C. songarica methanol extract on the kidney and heart tissue damage of albino rats, and to determine cytotoxic activity of various extracts of songarica on various human cancer cell lines.
Rats were divided into six groups, Group I received water only; Group II received CCl4 (1 mL/kg b wt) intraperitoneal; C. songarica extract (at doses of 100, 200 and 300 mg/kg b wt) orally for 15 days. Cytotoxic activity was determined by SRB method using MCF-7, HeLa, HepG2, SF-295, SW480 and IMR-32 cell lines.
Compared with CCl4 group, administration of C. songarica extract at the dose of 300 mg/kg b wt, significantly decreases serum creatinine (59.74%), urea (40.23%) and cholesterol (54 mg/dL), MDA (0.007 nmol/mg protein) in kidney and (0.025 nmol/mg protein) in heart tissue, along with evaluation of GSH (209.79 ± 54.6), GR (111.45 ± 2.84), GPx (94.01 ± 14.80), GST (201.71) in kidney tissue and GSH (51.47 ± 1.47), GR (45.42 ± 6.69), GPx (77.19 ± 10.94), GST (49.89) in heart tissue. In addition, methanol, ethanol and ethyl acetate extracts exhibited potent anticancer activity on six cancer cell lines with IC50 values ranging from 28.57 to 85.106 µg/mL.
Crataegus songarica methanol extract has a potential antioxidant effect as it protects the kidney and heart tissue against CCl4-induced toxicity, prevents DNA damage and showed strong anticancer activity.
准噶尔山楂（蔷薇科）已被用于民间医学治疗各种疾病。
本研究评估准噶尔山楂甲醇提取物对白化大鼠肾脏和心脏组织损伤的影响，并确定准噶尔山楂各种提取物对多种人类癌细胞系的细胞毒性活性。
将大鼠分为六组，第一组仅给予水；第二组腹腔注射四氯化碳（1 mL/kg体重）；准噶尔山楂提取物（剂量为100、200和300 mg/kg体重）口服给药15天。使用MCF-7、HeLa、HepG2、SF-295、SW480和IMR-32细胞系通过SRB法测定细胞毒性活性。
与四氯化碳组相比，以300 mg/kg体重剂量给予准噶尔山楂提取物，显著降低了血清肌酐（59.74%）、尿素（40.23%）和胆固醇（54 mg/dL），肾脏中丙二醛（0.007 nmol/mg蛋白质）和心脏组织中丙二醛（0.025 nmol/mg蛋白质），同时评估了肾脏组织中的谷胱甘肽（209.79 ± 54.6）、谷胱甘肽还原酶（111.45 ± 2.84）、谷胱甘肽过氧化物酶（94.01 ± 14.80）、谷胱甘肽S-转移酶（201.71）以及心脏组织中的谷胱甘肽（51.47 ± 1.47）、谷胱甘肽还原酶（45.42 ± 6.69）、谷胱甘肽过氧化物酶（77.19 ± 利用准噶尔山楂甲醇提取物对大鼠肾脏和心脏组织损伤的影响及细胞毒性活性研究
准噶尔山楂（蔷薇科）已被用于民间医学治疗各种疾病。
本研究评估准噶尔山楂甲醇提取物对白化大鼠肾脏和心脏组织损伤的影响，并确定准噶尔山楂各种提取物对多种人类癌细胞系的细胞毒性活性。
将大鼠分为六组，第一组仅给予水；第二组腹腔注射四氯化碳（1 mL/kg体重）；准噶尔山楂提取物（剂量为100、200和300 mg/kg体重）口服给药15天。使用MCF-7、HeLa、HepG2、SF-295、SW480和IMR-32细胞系通过SRB法测定细胞毒性活性。
与四氯化碳组相比，以300 mg/kg体重剂量给予准噶尔山楂提取物，显著降低了血清肌酐（59.74%）、尿素（40.23%）和胆固醇（54 mg/dL），肾脏中丙二醛（0.007 nmol/mg蛋白质）和心脏组织中丙二醛（0.025 nmol/mg蛋白质），同时评估了肾脏组织中的谷胱甘肽（209.79 ± 54.6）· 10.94）、谷胱甘肽S-转移酶（49.89）。此外，甲醇、乙醇和乙酸乙酯提取物对六种癌细胞系表现出强大的抗癌活性，IC50值范围为28.57至85.106 μg/mL。
准噶尔山楂甲醇提取物具有潜在的抗氧化作用，因为它可保护肾脏和心脏组织免受四氯化碳诱导的毒性，防止DNA损伤并表现出强大的抗癌活性。
