Domingos Helena Baruffaldi, Gonçalves Lucio Souza, de Uzeda Milton
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
Eur J Dent. 2015 Jul-Sep;9(3):411-414. doi: 10.4103/1305-7456.163225.
The present study is aimed to evaluate the antimicrobial action of Coltosol(®) in direct contact with human saliva.
Twelve different individuals were selected. Saliva samples were evaluated at four different time periods: Baseline 1 (T1-initial control), T2 (2 h), T4 (24 h after contact with a standardized sample of a coronary sealer) and baseline 2 (T3-final control). Seeded plates were incubated at 37°C in a bacterial incubator for a period of 48-72 h. After the incubation period, the colony forming units were counted, and the results compared.
Differences were statistically significant. There was an inhibition of bacterial growth after the first 2 h of contact and an increase in the number of bacteria after 24 h of direct contact between the material and the saliva. Coltosol(®) presented bacterial growth inhibition in direct contact with saliva. This inhibitory effect tended to decrease over time, as shown by the two periods when the material was in contact with different samples of saliva.
The antimicrobial activity of the material is an important feature; however, other physical and chemical properties of the coronary temporary sealer should be considered.
本研究旨在评估与人类唾液直接接触时Coltosol(®)的抗菌作用。
选取12名不同个体。在四个不同时间段对唾液样本进行评估：基线1（T1 - 初始对照）、T2（2小时）、T4（与冠状动脉封闭剂标准化样本接触24小时后）和基线2（T3 - 最终对照）。接种后的平板在37°C的细菌培养箱中孵育48 - 72小时。孵育期结束后，计数菌落形成单位并比较结果。
差异具有统计学意义。接触最初2小时后细菌生长受到抑制，而材料与唾液直接接触24小时后细菌数量增加。Coltosol(®)在与唾液直接接触时呈现出细菌生长抑制作用。随着时间推移，这种抑制作用趋于减弱，这在材料与不同唾液样本接触的两个时间段中有所体现。
该材料的抗菌活性是一个重要特性；然而，冠状动脉临时封闭剂的其他物理和化学性质也应予以考虑。
