抗阻训练期间摄入维生素D是否能改善青年和老年男性的骨骼肌肥大及力量反应？——一项随机对照试验。
Does vitamin-D intake during resistance training improve the skeletal muscle hypertrophic and strength response in young and elderly men? - a randomized controlled trial.
作者信息
Agergaard Jakob, Trøstrup Jeanette, Uth Jacob, Iversen Jonas Vestergard, Boesen Anders, Andersen Jesper L, Schjerling Peter, Langberg Henning
机构信息
Institute of Sports Medicine Copenhagen, Department of Orthopedic Surgery M, Bispebjerg Hospital and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
CopenRehab, Section of Social Medicine, Department of Public Health and Center for Healthy Aging, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
出版信息
Nutr Metab (Lond). 2015 Sep 30;12:32. doi: 10.1186/s12986-015-0029-y. eCollection 2015.
INTRODUCTION
Recent studies have shown that vitamin-D intake can improve skeletal muscle function and strength in frail vitamin-D insufficient individuals. We investigated whether vitamin-D intake can improve the muscular response to resistance training in healthy young and elderly individuals, respectively.
METHODS
Healthy untrained young (n = 20, age 20-30) and elderly (n = 20, age 60-75) men were randomized to 16 weeks of daily supplementary intake of either 48 μg of vitamin-D + 800 mg calcium (Vitamin-D-group) or 800 mg calcium (Placebo-group) during a period and at a latitude of low sunlight (December-April, 56°N). During the last 12 weeks of the supplementation the subjects underwent progressive resistance training of the quadriceps muscle. Muscle hypertrophy, measured as changes in cross sectional area (CSA), and isometric strength of the quadriceps were determined. Muscle biopsies were analyzed for fiber type morphology changes and mRNA expression of vitamin-D receptor (VDR), cytochrome p450 27B1 (CYP27B1) and Myostatin.
RESULTS
In the vitamin-D groups, serum 25(OH)D concentration increased significantly and at week 12 was significantly different from placebo in both young men (71.6 vs. 50.4 nmol/L, respectively) and elderly men (111.2 vs. 66.7 nmol/L, respectively). After 12 weeks of resistance training, quadriceps CSA and isometric strength increased compared to baseline in young (CSA p < 0.0001, strength p = 0.005) and elderly (CSA p = 0.001, strength p < 0.0001) with no difference between vitamin-D and placebo groups. Vitamin-D intake and resistance training increased strength/CSA in elderly compared to young (p = 0.008). In the young vitamin-D group, the change in fiber type IIa percentage was greater after 12 weeks training (p = 0.030) and Myostatin mRNA expression lower compared to the placebo group (p = 0.006). Neither resistance training nor vitamin-D intake changed VDR mRNA expression.
CONCLUSION
No additive effect of vitamin-D intake during 12 weeks of resistance training could be detected on either whole muscle hypertrophy or muscle strength, but improved muscle quality in elderly and fiber type morphology in young were observed, indicating an effect of vitamin-D on skeletal muscle remodeling.
TRIAL REGISTRATION
ClinicalTrials with nr. NCT01252381.
引言
最近的研究表明，维生素D摄入可改善维生素D不足的体弱个体的骨骼肌功能和力量。我们分别研究了维生素D摄入是否能改善健康年轻人和老年人对阻力训练的肌肉反应。
方法
健康的未经训练的年轻男性（n = 20，年龄20 - 30岁）和老年男性（n = 20，年龄60 - 75岁）被随机分为两组，在低日照时期（12月至4月，北纬56°），一组每天补充48μg维生素D + 800mg钙（维生素D组），另一组每天补充800mg钙（安慰剂组），为期16周。在补充的最后12周，受试者接受股四头肌的渐进性阻力训练。测定肌肉肥大情况（以横截面积（CSA）变化衡量）和股四头肌的等长力量。对肌肉活检样本进行分析，以检测纤维类型形态变化以及维生素D受体（VDR）、细胞色素P450 27B1（CYP27B1）和肌肉生长抑制素的mRNA表达。
结果
在维生素D组中，血清25(OH)D浓度显著升高，在第12周时，年轻男性（分别为71.6 vs. 50.4 nmol/L）和老年男性（分别为111.2 vs. 66.7 nmol/L）的血清25(OH)D浓度与安慰剂组相比均有显著差异。经过12周的阻力训练后，年轻组（CSA p < 0.0001，力量p = 0.005）和老年组（CSA p = 0.001，力量p < 0.0001）的股四头肌CSA和等长力量相较于基线均有所增加，维生素D组和安慰剂组之间无差异。与年轻组相比，维生素D摄入和阻力训练使老年组的力量/CSA增加（p = 0.008）。在年轻维生素D组中，训练12周后IIa型纤维百分比变化更大（p = 0.030），且肌肉生长抑制素mRNA表达低于安慰剂组（p = 0.006）。阻力训练和维生素D摄入均未改变VDR mRNA表达。
结论
在12周的阻力训练期间，未检测到维生素D摄入对全肌肉肥大或肌肉力量有附加作用，但观察到老年组肌肉质量改善以及年轻组纤维类型形态改变，表明维生素D对骨骼肌重塑有影响。
试验注册
ClinicalTrials编号为NCT01252381。
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