Agarwal Shivangi, Varma Dileep
a Department of Cell and Molecular Biology ; Feinberg School of Medicine; Northwestern University ; Chicago , IL USA.
Bioarchitecture. 2015;5(1-2):1-12. doi: 10.1080/19490992.2015.1090669. Epub 2015 Oct 2.
Mitosis entails the bona fide segregation of duplicated chromosomes. This process is accomplished by the attachment of kinetochores on chromosomes to microtubules (MTs) of the mitotic spindle. Once the appropriate attachment is achieved, the spindle assembly checkpoint (SAC) that delays the premature onset of anaphase needs to be silenced for the cell to proceed to anaphase and cytokinesis. Therefore, while it is imperative to preserve the SAC when kinetochores are unattached, it is of paramount importance that SAC components are removed post kinetochore microtubule (kMT) attachment. Precise knowledge of how kMT attachments trigger the removal of SAC components from kinetochores or how the checkpoint proteins feedback in to the attachment machinery remains elusive. This review aims to describe the recent advances that provide an insight into the interplay of molecular events that coordinate and regulate the SAC activity in response to kMT attachment during cell division.
有丝分裂需要将复制后的染色体进行真正的分离。这一过程通过染色体上的动粒与有丝分裂纺锤体的微管（MT）附着来完成。一旦实现了适当的附着，为延迟后期过早开始的纺锤体组装检查点（SAC）就需要被关闭，以便细胞进入后期和胞质分裂。因此，当动粒未附着时，保留SAC至关重要，但动粒微管（kMT）附着后去除SAC组件则至关重要。关于kMT附着如何触发SAC组件从动粒上移除，或者检查点蛋白如何反馈到附着机制，目前仍不清楚。本综述旨在描述最近的进展，这些进展有助于深入了解在细胞分裂过程中，响应kMT附着而协调和调节SAC活性的分子事件之间的相互作用。
