Wolpe S D, Sherry B, Juers D, Davatelis G, Yurt R W, Cerami A
Laboratory of Medical Biochemistry, Rockefeller University, New York, NY 10021-6399.
Proc Natl Acad Sci U S A. 1989 Jan;86(2):612-6. doi: 10.1073/pnas.86.2.612.
In response to endotoxin, macrophages secrete a protein with a molecular mass of approximately 6000 Da and with an affinity for heparin. This protein, which we term "macrophage inflammatory protein 2," is a potent chemotactic agent for human polymorphonuclear leukocytes. In addition, subcutaneous administration of the monokine causes a localized inflammatory reaction. Partial N-terminal sequence data reveal similarity to a family of proteins, the archetype of which is platelet factor 4. Although macrophage inflammatory protein 2 is a distinct member of the platelet factor 4 family, its sequence is most closely related to that of the gro/KC gene product, which is expressed in transformed or platelet-derived growth factor-treated cells.
巨噬细胞对内毒素产生反应时，会分泌一种分子量约为6000道尔顿且对肝素具有亲和力的蛋白质。我们将这种蛋白质称为“巨噬细胞炎性蛋白2”，它是人类多形核白细胞的一种强效趋化剂。此外，皮下注射这种单核因子会引起局部炎症反应。部分N端序列数据显示其与一类蛋白质相似，这类蛋白质的原型是血小板因子4。虽然巨噬细胞炎性蛋白2是血小板因子4家族的一个独特成员，但其序列与gro/KC基因产物的序列关系最为密切，gro/KC基因产物在转化细胞或血小板衍生生长因子处理的细胞中表达。
