Proteomics and Microbiology Department, Université de Mons, 20 place du Parc, 7000 Mons, Belgium; Electromagnetism and Telecommunication Department, Université de Mons, Bld Dolez 31, 7000 Mons, Belgium.
Proteomics and Microbiology Department, Université de Mons, 20 place du Parc, 7000 Mons, Belgium.
Biosens Bioelectron. 2017 Jun 15;92:449-456. doi: 10.1016/j.bios.2016.10.081. Epub 2016 Oct 29.
This work presents the development of an innovative plasmonic optical fiber (OF) immunosensor for the detection of cytokeratin 17 (CK17), a biomarker of interest for lung cancer diagnosis. The development of this sensing platform is such that it can be assessed in non-liquid environments, demonstrating that a surface plasmon resonance (SPR) can be excited in this case. For this purpose, detections have been first carried out on CK17 encapsulated in gel matrix in the aim of mimicking tissue samples. Gold-coated OF immunosensors were embedded in a specifically designed packaging providing enough stiffness to penetrate into soft matters. Resulting reflected spectra have revealed, for the first time, the presence of a stable SPR signal recorded in soft matters. Experiments conducted to detect CK17 trapped in a porous polyacrylamide gel matrix have highlighted the specific and selective biosensor response towards the target protein. Finally, the packaged OF immunosensor has been validated by a preliminary test on human lung biopsy, which has confirmed the ex-vivo CK17 detection. Consequently, this work represents an important milestone towards the detection of biomarkers in tissues, which is still a clinical challenge for minimally-invasive in vivo medical diagnosis.
这项工作提出了一种创新的等离子体光学纤维（OF）免疫传感器的发展，用于检测细胞角蛋白 17（CK17），这是肺癌诊断中感兴趣的生物标志物。该传感平台的开发可以在非液体环境中进行评估，证明在这种情况下可以激发出表面等离子体共振（SPR）。为此，首先在凝胶基质中封装 CK17 进行检测，旨在模拟组织样本。金包被的 OF 免疫传感器被嵌入到一个专门设计的包装中，提供足够的刚性以穿透软物质。所得反射光谱首次揭示了在软物质中记录到的稳定 SPR 信号的存在。在多孔聚丙烯酰胺凝胶基质中捕获 CK17 的实验突出了生物传感器对目标蛋白的特异性和选择性响应。最后，通过对人肺活检的初步测试验证了封装的 OF 免疫传感器，该测试证实了 CK17 的离体检测。因此，这项工作代表了在组织中检测生物标志物方面的一个重要里程碑，这仍然是体内微创医学诊断的一个临床挑战。
