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越南绿微藻HB高细胞生产力和虾青素积累的培养条件优化及其虾青素的神经保护活性

Optimization of Culture Conditions for High Cell Productivity and Astaxanthin Accumulation in Vietnam's Green Microalgae HB and a Neuroprotective Activity of Its Astaxanthin.

作者信息

Ha Nguyen Cam, Tam Luu Thi, Hien Hoang Thi Minh, Thu Ngo Thi Hoai, Hong Dang Diem, Thom Le Thi

机构信息

Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi 10000, Vietnam.

Faculty of Biotechnology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), Hanoi 10000, Vietnam.

出版信息

Bioengineering (Basel). 2024 Nov 21;11(12):1176. doi: 10.3390/bioengineering11121176.

DOI:10.3390/bioengineering11121176
PMID:39767994
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11672925/
Abstract

BACKGROUND

, a green microalga, is a rich source of natural astaxanthin and a potent antioxidant with high commercial value. This study investigates the biological characteristics and potential of HB isolated from Hoa Binh, Vietnam, for growth and astaxanthin accumulation using a two-phase culture method.

METHODS

HB was cultured in a C/RM medium at 25 °C, and morphological characteristics were examined. NMR spectroscopy was used to determine the structure of the astaxanthin, which was extracted using the Soxhlet method.

RESULTS

After 22 days, the highest cell density (4.96 × 10 cells mL) was achieved under optimized light and ultraviolet conditions. Nutrient deprivation followed by bicarbonate supplementation resulted in a maximal astaxanthin accumulation of 48.8 mg g dry cell weight within two days. The extracted astaxanthin demonstrated potent antioxidant activity (IC: 3.74 mg mL) compared to ascorbic acid (IC: 18.53 µg mL) and exhibited strong acetylcholinesterase inhibition (IC: 297.99 µg mL). It also showed neuroprotective effects against HO and amyloid beta-induced neurotoxicity in C6 cells.

CONCLUSIONS

This study highlights HB as a promising source for large-scale astaxanthin production with potential applications in neuroprotective health products.

摘要

背景

，一种绿色微藻，是天然虾青素的丰富来源，也是具有高商业价值的强效抗氧化剂。本研究采用两相培养法，研究了从越南和平分离的HB的生物学特性及其生长和虾青素积累的潜力。

方法

将HB在C/RM培养基中于25°C培养，并检查其形态特征。采用核磁共振光谱法测定虾青素的结构，并用索氏提取法提取虾青素。

结果

在优化的光照和紫外线条件下，22天后达到最高细胞密度（4.96×10个细胞/毫升）。营养剥夺后补充碳酸氢盐，在两天内虾青素最大积累量达到48.8毫克/克干细胞重量。与抗坏血酸（IC：18.53微克/毫升）相比，提取的虾青素表现出强效抗氧化活性（IC：3.74毫克/毫升），并表现出较强的乙酰胆碱酯酶抑制作用（IC：297.99微克/毫升）。它还对C6细胞中过氧化氢和淀粉样β蛋白诱导的神经毒性具有神经保护作用。

结论

本研究突出了HB作为大规模生产虾青素的有前途的来源，在神经保护健康产品中具有潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/9e2b934ffd01/bioengineering-11-01176-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/8ae41e3c993b/bioengineering-11-01176-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/16e97f85d156/bioengineering-11-01176-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/e9ba9ca55cce/bioengineering-11-01176-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/882cd2fe90bc/bioengineering-11-01176-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/98b9ec6293ff/bioengineering-11-01176-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/634f3f284c33/bioengineering-11-01176-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/3f36206281ab/bioengineering-11-01176-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/9e2b934ffd01/bioengineering-11-01176-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/8ae41e3c993b/bioengineering-11-01176-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/16e97f85d156/bioengineering-11-01176-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/e9ba9ca55cce/bioengineering-11-01176-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/882cd2fe90bc/bioengineering-11-01176-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/98b9ec6293ff/bioengineering-11-01176-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/634f3f284c33/bioengineering-11-01176-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/3f36206281ab/bioengineering-11-01176-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab20/11672925/9e2b934ffd01/bioengineering-11-01176-g008.jpg

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