State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China.
Department of Chemical and Materials Engineering, National I-Lan University, I-Lan 260, Taiwan.
Bioresour Technol. 2015 Dec;198:571-6. doi: 10.1016/j.biortech.2015.09.075. Epub 2015 Sep 26.
This study aimed to quantitatively evaluate the inhibitor tolerance of butanol-producing bacterium Clostridium acetobutylicum. The inhibitory effect of the inhibitors generated by acid pretreatment of biomass feedstock on butanol fermentation decreased in the order of formic acid>oxalic acid>furfural>5-HMF>Na2SO4. C. acetobutylicum has a small tolerance range for furfural (1.06-2.6g/L) and 5-HMF (1.99-2.3g/L). However, the inhibitory effect of Na2SO4 appears to have a wide range, with a chronic toxicity for C. acetobutylicum. All the results could explain, in quantitative manner, the instability of butanol fermentation with C. acetobutylicum from various acid-pretreated feedstocks caused by the fermentation inhibitors.
本研究旨在定量评估丁醇生产菌丙酮丁醇梭菌对抑制剂的耐受性。生物质原料酸预处理产生的抑制剂对丁醇发酵的抑制作用按甲酸>草酸>糠醛>5-HMF>Na2SO4的顺序依次降低。丙酮丁醇梭菌对糠醛（1.06-2.6g/L）和 5-HMF（1.99-2.3g/L）的耐受性范围较小。然而，Na2SO4 的抑制作用似乎范围较宽，对丙酮丁醇梭菌具有慢性毒性。所有结果都可以定量解释，由发酵抑制剂引起的各种酸预处理原料中，丙酮丁醇梭菌丁醇发酵不稳定的原因。
