Key Laboratory of Agricultural Microbial Enzyme Engineering (Ministry of Agriculture), College of Life Science, Henan Agricultural University, No. 63, Nongye Road, Jinshui District, Zhengzhou, 450002, Henan Province, China.
Centre for Agriculture and the Bioeconomy, Institute for Future Environments, Queensland University of Technology, Brisbane, QLD, 4000, Australia.
Sci Rep. 2020 Oct 21;10(1):17956. doi: 10.1038/s41598-020-74899-9.
In this study, the role of CaCO in n-butanol production was further investigated using corn straw hydrolysate (CSH) media by Clostridium acetobutylicum CICC 8016. CaCO addition stimulated sugars utilization and butanol production. Further study showed that calcium salts addition to CSH media led to the increase in Ca concentration both intracellularly and extracellularly. Interestingly, without calcium salts addition, intracellular Ca concentration in the synthetic P2 medium was much higher than that in the CSH medium despite the lower extracellular Ca concentrations in the P2 medium. These results indicated that without additional calcium salts, Ca uptake by C. acetobutylicum CICC 8016 in the CSH medium may be inhibited by non-sugar biomass degradation compounds, such as furans, phenolics and organic acids. Comparative proteomics analysis results showed that most enzymes involved in glycolysis, redox balance and amino acids metabolism were up-regulated with CaCO addition. This study provides further insights into the role of CaCO in n-butanol production using real biomass hydrolysate.
在这项研究中，进一步使用玉米秸秆水解物（CSH）培养基通过丙酮丁醇梭菌 CICC 8016 研究了 CaCO 在正丁醇生产中的作用。CaCO 添加刺激了糖的利用和丁醇的生产。进一步的研究表明，钙盐添加到 CSH 培养基中导致细胞内和细胞外 Ca 浓度的增加。有趣的是，即使在 P2 培养基中细胞外 Ca 浓度较低，没有添加钙盐时，合成 P2 培养基中的细胞内 Ca 浓度也远高于 CSH 培养基。这些结果表明，在没有额外的钙盐的情况下，CSH 培养基中 C. acetobutylicum CICC 8016 的 Ca 摄取可能受到非糖生物质降解化合物（如呋喃、酚类和有机酸）的抑制。比较蛋白质组学分析结果表明，大多数参与糖酵解、氧化还原平衡和氨基酸代谢的酶都随着 CaCO 的添加而上调。这项研究为使用真实生物质水解物生产正丁醇中 CaCO 的作用提供了进一步的见解。
