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利用白米糠进行同步糖化和发酵的开放式发酵生产 L-乳酸。

Open fermentative production of L-lactic acid using white rice bran by simultaneous saccharification and fermentation.

机构信息

National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.

National Energy R&D Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, PR China.

出版信息

Bioresour Technol. 2015 Dec;198:664-72. doi: 10.1016/j.biortech.2015.09.010. Epub 2015 Sep 25.

Abstract

To reduce raw material cost for lactic acid production, white rice bran as an important byproduct in rice milling, was used in l-lactic acid production by open simultaneous saccharification and fermentation (SSF). Although one thermotolerant strain was used at a temperature as high as 50°C, the open fermentation was still inefficient due to the indigenous thermophilic bacteria from corn steep liquor powder. A stepwise controlled pH was proposed in open SSF process, and no complicated pretreatment or sterilization was needed before fermentation. In batch fermentation, 117 gL(-1) lactic acid was obtained, and the productivity and yield reached 2.79 gL(-1) h(-1) and 98.75%, respectively. These results showed an efficient way to develop high value-added products from white rice bran.

摘要

为降低乳酸生产成本,采用糙米糠这一稻米加工的重要副产物,通过敞开式同步糖化发酵(SSF)生产 L-乳酸。尽管使用了一株耐热菌,发酵温度高达 50°C,但由于来自玉米浆干粉的嗜热菌,敞开式发酵仍然效率低下。在敞开式 SSF 过程中提出了分步控制 pH 值的方法,发酵前无需进行复杂的预处理或灭菌。在分批发酵中,获得了 117 g/L 的乳酸,产物得率和生产效率分别达到 2.79 g/L·h 和 98.75%。这些结果表明了一种从糙米糠开发高附加值产品的有效方法。

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