Ojeda M L, Rua R M, Nogales F, Díaz-Castro J, Murillo M L, Carreras O
Department of Physiology, Faculty of Pharmacy, Seville University, Seville 41012, Spain.
Department of Physiology, Faculty of Pharmacy and Institute of Nutrition and Food Technology 'José Mataix', University of Granada, Granada 18071, Spain.
Alcohol Alcohol. 2016 May;51(3):235-41. doi: 10.1093/alcalc/agv111. Epub 2015 Oct 3.
An important mechanism in alcohol-induced injury is biomolecular oxidative damage. Folic acid is supplied to chronic alcoholic patients in order to prevent this situation, as this is the main vitamin deficiency that they suffer from. Acute alcohol exposure, such as binge drinking, is one of the most widespread ethanol consumption models practiced by adolescents. However, there is no evidence of folic acid body profiles after this pattern of consumption.
Four groups of adolescent rats were used: control, alcohol (exposed to intraperitoneal binge drinking), control folic acid-supplemented group and alcohol folic acid-supplemented group. Folic acid levels, protein, lipid and DNA oxidative damage in serum, and liver glutathione (GSH) and reduced/oxidized glutathione ratio (GSH/GSSG) were measured.
Binge-drinking rats had higher lipids and DNA oxidation levels. They also had lower hepatic GSH levels and GSH/GSSG ratio. Folic acid supplementation to binge-drinking rats does not change the serum protein oxidation but decreases lipid and DNA oxidation. Finally, GSH increased to control levels with folic acid supplementation.
Folic acid supplementation is an economic and efficient therapy against the oxidative damage in lipids and mainly in DNA stability caused by binge drinking during adolescence. It has also been demonstrated that folic acid increases GSH levels, improving the antioxidant status and revealing a hepatoprotective effect during binge drinking.
酒精诱导损伤的一个重要机制是生物分子氧化损伤。向慢性酒精中毒患者补充叶酸以预防这种情况，因为这是他们主要缺乏的维生素。急性酒精暴露，如暴饮，是青少年中最普遍的乙醇消费模式之一。然而，没有证据表明这种消费模式后叶酸的体内情况。
使用四组青春期大鼠：对照组、酒精组（腹腔内暴饮）、对照补充叶酸组和酒精补充叶酸组。测量血清中的叶酸水平、蛋白质、脂质和DNA氧化损伤，以及肝脏谷胱甘肽（GSH）和还原/氧化型谷胱甘肽比率（GSH/GSSG）。
暴饮大鼠的脂质和DNA氧化水平较高。它们的肝脏GSH水平和GSH/GSSG比率也较低。给暴饮大鼠补充叶酸不会改变血清蛋白质氧化，但会降低脂质和DNA氧化。最后，补充叶酸后GSH增加到对照水平。
补充叶酸是一种经济有效的疗法，可对抗青少年暴饮引起的脂质氧化损伤，主要是DNA稳定性损伤。还证明叶酸可提高GSH水平，改善抗氧化状态，并在暴饮期间显示出肝脏保护作用。
