School of Material Science and Engineering, Lanzhou University of Technology, Gansu Lanzhou 730050, China; State Key Laboratory of Gansu Advanced Non-Ferrous Metal Materials, Lanzhou University of Technology, Gansu Lanzhou 730050, China.
School of Material Science and Engineering, Lanzhou University of Technology, Gansu Lanzhou 730050, China; State Key Laboratory of Gansu Advanced Non-Ferrous Metal Materials, Lanzhou University of Technology, Gansu Lanzhou 730050, China; Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences, Lanzhou 730000, China.
Food Chem. 2012 May 1;132(1):268-76. doi: 10.1016/j.foodchem.2011.10.077. Epub 2011 Oct 31.
The separation and purification of Rebaudioside A (RA) from Stevia rebaudiana Bertoni crude extracts (Steviosides) by macroporous adsorption resin (MAR) mixed bed were systematically investigated. MAR mixed bed of HPD750-LSA40-LSA30-DS401 was selected due to its better separation degree. Based on the kinetics/thermodynamics experiment of the mixed bed, it was found that the experimental data fitted better to the pseudo-second-order model, and intra-particle diffusion was rate-limiting step. The adsorption isotherm was consistent with IV equilibrium adsorption isotherm classified by Brunauer. Furthermore, the influencing factors for the separation of RA based on HPLC were also investigated. Under the optimal conditions, the separation degree for RA (DAS) increased from 0.771 to 1.54. Moreover, the experimental results showed that the purity of the obtained product increased from 60% to 97%.
通过大孔吸附树脂（MAR）混合床系统地研究了从甜菊叶粗提物（甜菊糖苷）中分离和纯化莱鲍迪甙 A（RA）。由于具有更好的分离度，选择了 HPD750-LSA40-LSA30-DS401 MAR 混合床。基于混合床的动力学/热力学实验，发现实验数据更符合拟二阶模型，并且内扩散是速率限制步骤。吸附等温线与 Brunauer 分类的 IV 平衡吸附等温线一致。此外，还研究了基于 HPLC 的 RA 分离的影响因素。在最佳条件下，RA（DAS）的分离度从 0.771 增加到 1.54。此外，实验结果表明，所得产物的纯度从 60%提高到 97%。
