Zhang Qing, Wang Jie, Li Chanyuan, Zheng Miaoxin, He Zihan, Zou Yuting, Xiong Haibo, Xu Bitao, Xiang Wenliang, Tang Jie
Key Laboratory of Food Microbiology, School of Food and Bioengineering, Xihua University, Chengdu 610039, China.
Foods. 2022 Nov 15;11(22):3649. doi: 10.3390/foods11223649.
The characterization and bioactive properties of carotenoid produced by GH-1 originating from Pixian Douban (PXDB), the Chinese traditional condiment, was investigated. The produced and purified yellow pigment was characterized by ultraviolet-visible spectroscopy (UV-Vis), Fourier transformed infrared (FTIR), nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS), and was identified as carotenoid lutein. Additionally, the bioactive activity of lutein from GH-1 was evaluated by measuring the free radical scavenging capacity in vitro and feeding zebrafish lutein through aqueous solution. The results showed that the carotenoid lutein had strong antioxidant capacity and a protective effect on zebrafish eye cells, which could inhibit the apoptosis of eye cells in a concentration dependent manner. The results suggested that carotenoid lutein from GH-1 could be utilized as a potential source of natural antioxidants or functional additives for food/pharmaceutical industries.
对源自中国传统调味品郫县豆瓣（PXDB）的GH-1所产类胡萝卜素的特性及生物活性进行了研究。通过紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FTIR）、核磁共振（NMR）和高分辨率质谱（HRMS）对所产并纯化的黄色色素进行了表征，鉴定其为类胡萝卜素叶黄素。此外，通过体外测定自由基清除能力以及通过水溶液向斑马鱼投喂叶黄素，评估了GH-1来源的叶黄素的生物活性。结果表明，类胡萝卜素叶黄素具有较强的抗氧化能力，对斑马鱼眼细胞具有保护作用，能够以浓度依赖的方式抑制眼细胞凋亡。结果表明，GH-1来源的类胡萝卜素叶黄素可作为食品/制药行业潜在的天然抗氧化剂或功能性添加剂来源。
