Krejčová Anna, Ludvíková Iveta, Černohorský Tomáš, Pouzar Miloslav
Department of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic.
Department of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic.
Food Chem. 2012 May 1;132(1):588-96. doi: 10.1016/j.foodchem.2011.10.076. Epub 2011 Oct 31.
This paper reports the development and using of ICP-MS and ICP-OES methods for determination of major, minor and trace elements in multivitamin preparations and dietary supplements and, based on results, their classification using multivariate statistical methods. The method was optimised and evaluated with the use of "in-house" reference material, commercial reference materials and spiked samples. Macroelements (Na, K, Ca, Mg, P), microelements (Cu, Fe, Mn, Zn, Se, Cr), toxic elements (As, Cd, Pb, Ni, V) and a whole series of other elements (e.g. REEs, Ti, Au, Pt, Pd, etc.) were detected with excellent limits of detection as well as other procedure parameters suitable for the purpose of the elemental analysis. As for physiologically significant elements, contents were found as follows (mgkg(-1)): Ca 560-196,000, Mg 308-70,300, Na 289-74,000, K from undetectable to 28,200, Cu 28-1460, P from undetectable to 217,000, Zn 2.1-25,000, Fe 115-39,400, Mn 28-1860, Cr 0.486-26.6, Mo 0.0418-9.88, and Se 0.395-161. It was found out, if compared with recommended daily intakes, that maximally 50% Ca, 37% Mg, 3% K, 20% P, 14% Na, 100% Cu, 160% Mn, 190% Fe, 120% Zn, 24% Cr, 29% Mo and 79% Se can be obtained from one dose. Extreme samples presenting a possible health risk (Ni content nearly 200mgkg(-1)) were indicated. Most of the remaining elements (e.g. REEs) were analysed in order to get reference values for nutritional preparation.
本文报道了电感耦合等离子体质谱法（ICP-MS）和电感耦合等离子体发射光谱法（ICP-OES）在多种维生素制剂和膳食补充剂中常量、微量和痕量元素测定方面的开发与应用，并基于测定结果，采用多元统计方法对其进行分类。该方法通过使用“内部”参考物质、商业参考物质和加标样品进行了优化和评估。检测了常量元素（钠、钾、钙、镁、磷）、微量元素（铜、铁、锰、锌、硒、铬）、有毒元素（砷、镉、铅、镍、钒）以及一系列其他元素（如稀土元素、钛、金、铂、钯等），检测限极佳，且其他程序参数也适用于元素分析目的。至于具有生理意义的元素，其含量如下（毫克/千克）：钙560 - 196,000、镁308 - 70,300、钠289 - 74,000、钾未检出至28,200、铜28 - 1460、磷未检出至217,000、锌2.1 - 25,000、铁115 - 39,400、锰28 - 1860、铬0.486 - 26.6、钼0.04l8 - 9.88、硒0.395 - 161。研究发现，与推荐的每日摄入量相比，一次剂量中最多可获取50%的钙、37%的镁、3%的钾、20%的磷、14%的钠、100%的铜、160%的锰、190%的铁、120%的锌、24%的铬、29%的钼和79%的硒。指出了存在可能健康风险的极端样品（镍含量接近200毫克/千克）。对大多数其余元素（如稀土元素）进行了分析，以获取营养制剂的参考值。
