Sharma Om P, Kumar Neeraj, Singh Bikram, Bhat Tej K
Biochemistry Laboratory, Indian Veterinary Research Institute, Regional Station, Palampur 176 061, Himachal Pradesh, India.
Division of Natural Plant Products, Institute of Himalayan Bioresource Technology, Palampur 176 061, Himachal Pradesh, India.
Food Chem. 2012 May 1;132(1):671-4. doi: 10.1016/j.foodchem.2011.10.069. Epub 2011 Oct 28.
Analysis of saponins by thin layer chromatography (TLC) is reported. The solvent system was n-butanol:water:acetic acid (84:14:7). Detection of saponins on the TLC plates after development and air-drying was done by immersion in a suspension of sheep erythrocytes, followed by washing off the excess blood on the plate surface. Saponins appeared as white spots against a pink background. The protocol provided specific detection of saponins in the saponins enriched extracts from Aesculusindica (Wall. ex Camb.) Hook.f., Lonicera japonica Thunb., Silene inflata Sm., Sapindusmukorossi Gaertn., Chlorophytum borivilianum Santapau & Fernandes, Asparagusadscendens Roxb., Asparagus racemosus Willd., Agave americana L., Camellia sinensis [L.] O. Kuntze. The protocol is convenient, inexpensive, does not require any corrosive chemicals and provides specific detection of saponins.
报道了用薄层色谱法（TLC）分析皂苷的方法。溶剂系统为正丁醇：水：乙酸（84:14:7）。展开并晾干后的TLC板上的皂苷检测，是通过将其浸入绵羊红细胞悬液中，然后洗去板表面多余的血液来进行的。皂苷在粉红色背景下呈现为白色斑点。该方法可特异性检测印度七叶树（Wall. ex Camb.）Hook.f.、忍冬、胀萼蝇子草、无患子、波维吊兰、攀援天门冬、总状天门冬、龙舌兰、茶树[L.] O. Kuntze等富含皂苷提取物中的皂苷。该方法简便、成本低，不需要任何腐蚀性化学物质，且能特异性检测皂苷。
