Keerthikumar Shivakumar, Chisanga David, Ariyaratne Dinuka, Al Saffar Haidar, Anand Sushma, Zhao Kening, Samuel Monisha, Pathan Mohashin, Jois Markandeya, Chilamkurti Naveen, Gangoda Lahiru, Mathivanan Suresh
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia.
Department of Computer Science and Information Technology, La Trobe University, Melbourne, VIC 3086, Australia.
J Mol Biol. 2016 Feb 22;428(4):688-692. doi: 10.1016/j.jmb.2015.09.019. Epub 2015 Oct 3.
Exosomes are membranous vesicles that are released by a variety of cells into the extracellular microenvironment and are implicated in intercellular communication. As exosomes contain RNA, proteins and lipids, there is a significant interest in characterizing the molecular cargo of exosomes. Here, we describe ExoCarta (http://www.exocarta.org), a manually curated Web-based compendium of exosomal proteins, RNAs and lipids. Since its inception, the database has been highly accessed (>54,000 visitors from 135 countries). The current version of ExoCarta hosts 41,860 proteins, >7540 RNA and 1116 lipid molecules from more than 286 exosomal studies annotated with International Society for Extracellular Vesicles minimal experimental requirements for definition of extracellular vesicles. Besides, ExoCarta features dynamic protein-protein interaction networks and biological pathways of exosomal proteins. Users can download most often identified exosomal proteins based on the number of studies. The downloaded files can further be imported directly into FunRich (http://www.funrich.org) tool for additional functional enrichment and interaction network analysis.
外泌体是由多种细胞释放到细胞外微环境中的膜性囊泡，参与细胞间通讯。由于外泌体含有RNA、蛋白质和脂质，因此人们对表征外泌体的分子货物有着浓厚的兴趣。在这里，我们描述了ExoCarta（http://www.exocarta.org），这是一个基于网络的人工整理的外泌体蛋白质、RNA和脂质的汇编。自创建以来，该数据库的访问量很大（来自135个国家的访问者超过54000人）。ExoCarta的当前版本收录了来自286多项外泌体研究的41860种蛋白质、超过7540种RNA和1116种脂质分子，这些研究都标注了国际细胞外囊泡协会对细胞外囊泡定义的最低实验要求。此外，ExoCarta还具有外泌体蛋白质的动态蛋白质-蛋白质相互作用网络和生物学途径。用户可以根据研究数量下载最常鉴定出的外泌体蛋白质。下载的文件可以进一步直接导入FunRich（http://www.funrich.org）工具，进行额外的功能富集和相互作用网络分析。
