Memorial Sloan Kettering Cancer Center, Molecular Pharmacology & Chemistry, 1275 York Avenue, New York, NY 10065, USA ; Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
Basic Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, P.O. Box 9530, Morgantown, WV 26506, USA.
J Immunol Res. 2015;2015:186586. doi: 10.1155/2015/186586. Epub 2015 Sep 7.
Differential cell systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro selection method for obtaining molecular recognition elements (MREs) that specifically bind to individual cell types with high affinity. MREs are selected from initial large libraries of different nucleic or amino acids. This review outlines the construction of peptide and antibody fragment libraries as well as their different host types. Common methods of selection are also reviewed. Additionally, examples of cancer cell MREs are discussed, as well as their potential applications.
通过指数富集的配体系统进化差异（SELEX）是一种体外选择方法，用于获得分子识别元件（MREs），这些元件能够与特定的细胞类型以高亲和力特异性结合。MREs 是从不同核酸或氨基酸的初始大型文库中选择出来的。本文综述了肽和抗体片段文库的构建以及它们不同的宿主类型。还回顾了常见的选择方法。此外，还讨论了癌细胞 MREs 的实例及其潜在的应用。
