Arnold W David, Sheth Kajri A, Wier Christopher G, Kissel John T, Burghes Arthur H, Kolb Stephen J
Department of Neurology, The Ohio State University Wexner Medical Center; Department of Physical Medicine and Rehabilitation, The Ohio State University; Department of Neuroscience, The Ohio State University Wexner Medical Center;
Department of Neurology, The Ohio State University Wexner Medical Center.
J Vis Exp. 2015 Sep 25(103):52899. doi: 10.3791/52899.
Compound muscle action potential (CMAP) and motor unit number estimation (MUNE) are electrophysiological techniques that can be used to monitor the functional status of a motor unit pool in vivo. These measures can provide insight into the normal development and degeneration of the neuromuscular system. These measures have clear translational potential because they are routinely applied in diagnostic and clinical human studies. We present electrophysiological techniques similar to those employed in humans to allow recordings of mouse sciatic nerve function. The CMAP response represents the electrophysiological output from a muscle or group of muscles following supramaximal stimulation of a peripheral nerve. MUNE is an electrophysiological technique that is based on modifications of the CMAP response. MUNE is a calculated value that represents the estimated number of motor neurons or axons (motor control input) supplying the muscle or group of muscles being tested. We present methods for recording CMAP responses from the proximal leg muscles using surface recording electrodes following the stimulation of the sciatic nerve in mice. An incremental MUNE technique is described using submaximal stimuli to determine the average single motor unit potential (SMUP) size. MUNE is calculated by dividing the CMAP amplitude (peak-to-peak) by the SMUP amplitude (peak-to-peak). These electrophysiological techniques allow repeated measures in both neonatal and adult mice in such a manner that facilitates rapid analysis and data collection while reducing the number of animals required for experimental testing. Furthermore, these measures are similar to those recorded in human studies allowing more direct comparisons.
复合肌肉动作电位（CMAP）和运动单位数量估计（MUNE）是可用于在体内监测运动单位池功能状态的电生理技术。这些测量方法能够深入了解神经肌肉系统的正常发育和退化情况。由于它们在人类诊断和临床研究中常规应用，所以这些测量方法具有明确的转化潜力。我们介绍了与人类所采用的类似的电生理技术，以便记录小鼠坐骨神经功能。CMAP反应代表在对周围神经进行超强刺激后，一块肌肉或一组肌肉的电生理输出。MUNE是一种基于CMAP反应改良的电生理技术。MUNE是一个计算值，代表供应受试肌肉或一组肌肉的运动神经元或轴突（运动控制输入）的估计数量。我们介绍了在小鼠坐骨神经受到刺激后，使用表面记录电极记录近端腿部肌肉CMAP反应的方法。描述了一种递增式MUNE技术，该技术使用次最大刺激来确定平均单个运动单位电位（SMUP）大小。MUNE通过将CMAP幅度（峰峰值）除以SMUP幅度（峰峰值）来计算。这些电生理技术允许在新生小鼠和成年小鼠中进行重复测量，其方式便于快速分析和数据收集，同时减少实验测试所需的动物数量。此外，这些测量结果与人类研究中记录的结果相似，从而能够进行更直接的比较。
