Covarrubías Natalia, Hurtado Carmen, Díaz Alex, Mezzano Gabriel, Brahm Javier, Venegas Mauricio
Rev Chilena Infectol. 2015 Aug;32(4):482-4. doi: 10.4067/S0716-10182015000500020.
Reported seroprevalence of hepatitis E virus (HEV) in developed countries is between 0.3-53%. Published data relies on the assays used and its technical performance. Sensitivity on new available tests has improved, which has changed HEV seroprevalence around the world. We re-evaluated retrospectively, 178 serum samples of patients with previous anti HEV IgG determination between 2009 and 2012. Initial analysis was performed with ELISA kit Genelabs (Singapore), with 7.3% positivity. The reevaluation was done with ELISA kit AccuDiag TM HEV-IgG (Diagnostic Automation, United States), with reported sensitivity and specificity over 99.8%. With the new assay, 32.6% positive samples were found, significantly greater to the previous result (p<0.001) (4.5 times more). There were no differences in gender but a significant association between age and HEV IgG seropositivity was found (p<0.001). This suggests that previous testing might have underestimated HEV seroprevalence in Chile, which should be reevaluated using the new available test.
在发达国家，戊型肝炎病毒（HEV）的血清流行率报告为0.3%-53%。已发表的数据取决于所使用的检测方法及其技术性能。新型检测方法的敏感性有所提高，这改变了全球戊型肝炎病毒的血清流行率。我们对2009年至2012年间曾检测过抗HEV IgG的178例患者的血清样本进行了回顾性重新评估。最初使用新加坡Genelabs公司的ELISA试剂盒进行分析，阳性率为7.3%。重新评估使用美国Diagnostic Automation公司的AccuDiag TM HEV-IgG ELISA试剂盒，其报告的敏感性和特异性超过99.8%。使用新检测方法发现32.6%的样本呈阳性，显著高于之前的结果（p<0.001）（高出4.5倍）。性别之间没有差异，但发现年龄与HEV IgG血清阳性之间存在显著关联（p<0.001）。这表明之前的检测可能低估了智利戊型肝炎病毒的血清流行率，应使用新型检测方法重新评估。
