克罗地亚献血者中戊型肝炎检测相关血清流行率的估计。

Estimation of the hepatitis E assay-dependent seroprevalence among Croatian blood donors.

作者信息

Miletić M, Vuk T, Hećimović A, Stojić Vidović M, Jemeršić L, Jukić I

机构信息

Croatian Institute of Transfusion Medicine (CITM), Petrova 3, 10000 Zagreb, Croatia.

Croatian Institute of Transfusion Medicine (CITM), Petrova 3, 10000 Zagreb, Croatia; Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia.

出版信息

Transfus Clin Biol. 2019 Nov;26(4):229-233. doi: 10.1016/j.tracli.2019.06.234. Epub 2019 Jun 24.

DOI:10.1016/j.tracli.2019.06.234

PMID:31277986

Abstract

BACKGROUND

Seroprevalence of hepatitis E virus (HEV) in blood donors presenting to the Croatian Institute of Transfusion Medicine was assessed with 4 available tests (3 ELISA tests and 1 immunoblot (IB) test).

MATERIALS AND METHODS

In October and November 2014, a total of 1,036 serum samples of blood donors were collected for the study. Samples were primarily tested for total HEV antibodies by Dia.Pro HEV Ab test (a). All reactive samples were tested by ELISA tests: Dia.Pro HEV IgG (b) and IgM (c), Mikrogen recomWell HEV IgG_old (d) and IgM_old (e), recomWell HEV IgG_new (f) and IgM_new (g), and IB Mikrogen recomLine HEV IgG (h) and IgM (i). HEV IgM reactive samples also positive by the IB were further tested for HEV RNA.

RESULTS

There were 21.5% of samples reactive for total HEV antibodies (a). Seroprevalence of HEV IgG according to the b, d, f and h tests was 20.2%, 9.6%, 18.1% and 17.8%, respectively. Seroprevalence of HEV IgM according to the c, e, g and i tests was 4.4%, 1.5%, 2.0% and 1.7%, respectively. Out of 46 HEV IgM (Dia.Pro HEV IgM) positive samples, 18 (39.1%) were also positive by IB. HEV RNA was not detected in any of those samples. There was a significant association between age and HEV seroprevalence (P<0.001).

CONCLUSION

Different HEV antibody detection assays showed a high HEV IgG seroprevalence in Croatian blood donors. Among HEV IgG and HEV IgM positive samples HEV RNA was not detected.

摘要

背景

采用4种现有检测方法（3种酶联免疫吸附测定（ELISA）法和1种免疫印迹（IB）法）评估了前往克罗地亚输血医学研究所的献血者中戊型肝炎病毒（HEV）的血清流行率。

材料与方法

2014年10月和11月，共收集了1036份献血者的血清样本用于该研究。样本首先通过Dia.Pro HEV Ab检测（a）进行总HEV抗体检测。所有反应性样本均通过ELISA检测：Dia.Pro HEV IgG（b）和IgM（c）、Mikrogen recomWell HEV IgG_old（d）和IgM_old（e）、recomWell HEV IgG_new（f）和IgM_new（g），以及IB Mikrogen recomLine HEV IgG（h）和IgM（i）。HEV IgM反应性样本且IB检测也呈阳性的样本进一步检测HEV RNA。

结果

21.5%的样本总HEV抗体呈反应性（a）。根据b、d、f和h检测，HEV IgG的血清流行率分别为20.2%、9.6%、18.1%和17.8%。根据c、e、g和i检测，HEV IgM的血清流行率分别为4.4%、1.5%、2.0%和1.7%。在46份HEV IgM（Dia.Pro HEV IgM）阳性样本中，有18份（39.1%）IB检测也呈阳性。这些样本中均未检测到HEV RNA。年龄与HEV血清流行率之间存在显著关联（P<0.001）。