Sirimanapong Wanna, Thompson Kim D, Ooi Ei Lin, Bekaert Michaël, Collet Bertrand, Taggart John B, Bron James E, Green Darren M, Shinn Andrew P, Adams Alexandra, Leaver Michael J
Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK; Faculty of Veterinary Sciences, Mahidol University, Salaya Campus, Nakornpathom, Thailand.
Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK; Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Near Edinburgh, Scotland, UK.
Fish Shellfish Immunol. 2015 Nov;47(1):595-605. doi: 10.1016/j.fsi.2015.09.042. Epub 2015 Oct 9.
Pangasianodon hypophthalmus (striped catfish) is an important aquaculture species and intensification of farming has increased disease problems, particularly Edwardsiella ictaluri. The effects of feeding β-glucans on immune gene expression and resistance to E. ictaluri in P. hypophthalmus were explored. Fish were fed 0.1% fungal-derived β-glucan or 0.1% commercial yeast-derived β-glucan or a basal control diet without glucan. After 14 days of feeding, the mRNA expression of immune genes (transferrin, C-reactive protein, precerebellin-like protein, Complement C3 and factor B, 2a MHC class II and interleukin-1 beta) in liver, kidney and spleen were determined. Following this fish from each of the three diet treatment groups were infected with E. ictaluri and further gene expression measured 24 h post-infection (h.p.i.), while the remaining fish were monitored over 2 weeks for mortalities. Cumulative percentage mortality at 14 days post-infection (d.p.i.) was less in β-glucan fed fish compared to controls. There was no difference in gene expression between dietary groups after feeding for 14 days, but there was a clear difference between infected and uninfected fish at 24 h.p.i., and based on principal component analysis β-glucans stimulated the overall expression of immune genes in the liver, kidney and spleen at 24 h.p.i.
苏氏圆腹芒（条纹鲶鱼）是一种重要的水产养殖品种，养殖集约化增加了疾病问题，尤其是爱德华氏菌。本研究探讨了投喂β-葡聚糖对苏氏圆腹芒免疫基因表达及抗爱德华氏菌能力的影响。给鱼投喂0.1%的真菌源β-葡聚糖或0.1%的商业酵母源β-葡聚糖或不含葡聚糖的基础对照饲料。投喂14天后，测定肝脏、肾脏和脾脏中免疫基因（转铁蛋白、C反应蛋白、小脑前体样蛋白、补体C3和B因子、2a MHC II类分子和白细胞介素-1β）的mRNA表达。此后，将三个饮食处理组中的每组鱼感染爱德华氏菌，并在感染后24小时（h.p.i.）测定进一步的基因表达，而其余的鱼则在2周内监测死亡率。与对照组相比，投喂β-葡聚糖的鱼在感染后14天（d.p.i.）的累积死亡率较低。投喂14天后，不同饮食组之间的基因表达没有差异，但在感染后24小时，感染鱼和未感染鱼之间存在明显差异，基于主成分分析，β-葡聚糖在感染后24小时刺激了肝脏、肾脏和脾脏中免疫基因的整体表达。
