Nuruzzaman Mohammed, Sharoni Akhter M, Satoh Kouji, Karim Mohammad Rezaul, Harikrishna Jennifer A, Shimizu Takumi, Sasaya Takahide, Omura Toshihiro, Haque Mohammad A, Hasan Sayed M Z, Ahmad Aziz, Kikuchi Shoshi
Plant Genome Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences Tsukuba, Japan ; Faculty of Science, Centre for Research for Biotechnology for Agriculture, Institute of Biological Sciences, University of Malaya Kuala Lumpur, Malaysia ; Post Harvest Technology, School of Food Science and Technology, University Malaysia Terengganu Kuala Terengganu, Malaysia ; Department of Agronomy and Agricultural Extension, Faculty of Agriculture, University of Rajshahi Rajshahi, Bangladesh.
Plant Genome Research Unit, Agrogenomics Research Center, National Institute of Agrobiological Sciences Tsukuba, Japan.
Front Plant Sci. 2015 Sep 9;6:676. doi: 10.3389/fpls.2015.00676. eCollection 2015.
Expression levels of the NAC gene family were studied in rice infected with Rice dwarf virus (RDV), Rice black-streaked dwarf virus (RBSDV), Rice grassy stunt virus (RGSV), Rice ragged stunt virus (RRSV), and Rice transitory yellowing virus (RTYV). Microarray analysis showed that 75 (68%) OsNAC genes were differentially regulated during infection with RDV, RBSDV, RGSV, and RRSV compared with the control. The number of OsNAC genes up-regulated was highest during RGSV infection, while the lowest number was found during RTYV infection. These phenomena correlate with the severity of the syndromes induced by the virus infections. Most of the genes in the NAC subgroups NAC22, SND, ONAC2, ANAC34, and ONAC3 were down-regulated for all virus infections. These OsNAC genes might be related to the health stage maintenance of the host plants. Interestingly, most of the genes in the subgroups TIP and SNAC were more highly expressed during RBSDV and RGSV infections. These results suggested that OsNAC genes might be related to the responses induced by the virus infection. All of the genes assigned to the TIP subgroups were highly expressed during RGSV infection when compared with the control. For RDV infection, the number of activated genes was greatest during infection with the S-strain, followed by the D84-strain and the O-strain, with seven OsNAC genes up-regulated during infection by all three strains. The Os12g03050 and Os11g05614 genes showed higher expression during infection with four of the five viruses, and Os11g03310, Os11g03370, and Os07g37920 genes showed high expression during at least three viral infections. We identified some duplicate genes that are classified as neofunctional and subfunctional according to their expression levels in different viral infections. A number of putative cis-elements were identified, which may help to clarify the function of these key genes in network pathways.
对感染水稻矮缩病毒（RDV）、水稻黑条矮缩病毒（RBSDV）、水稻草状矮化病毒（RGSV）、水稻锯齿叶矮缩病毒（RRSV）和水稻暂黄病毒（RTYV）的水稻中NAC基因家族的表达水平进行了研究。微阵列分析表明，与对照相比，在感染RDV、RBSDV、RGSV和RRSV期间，75个（68%）OsNAC基因受到差异调节。在RGSV感染期间，上调的OsNAC基因数量最多，而在RTYV感染期间数量最少。这些现象与病毒感染引起的综合征严重程度相关。在所有病毒感染中，NAC亚组NAC22、SND、ONAC2、ANAC34和ONAC3中的大多数基因均下调。这些OsNAC基因可能与寄主植物的健康状态维持有关。有趣的是，在RBSDV和RGSV感染期间，TIP和SNAC亚组中的大多数基因表达更高。这些结果表明，OsNAC基因可能与病毒感染诱导的反应有关。与对照相比，所有分配到TIP亚组的基因在RGSV感染期间均高表达。对于RDV感染，在S株感染期间激活的基因数量最多，其次是D84株和O株，在所有三种毒株感染期间均有7个OsNAC基因上调。Os12g03050和Os11g05614基因在五种病毒中的四种感染期间表达较高，而Os11g03310、Os11g03370和Os07g37920基因在至少三种病毒感染期间表达较高。我们根据它们在不同病毒感染中的表达水平鉴定了一些重复基因，这些基因被分类为新功能基因和亚功能基因。鉴定出了一些推定的顺式元件，这可能有助于阐明这些关键基因在网络途径中的功能。
