用于软骨合成的剪切与压缩生物反应器。

Shear and Compression Bioreactor for Cartilage Synthesis.

作者信息

Shahin Kifah, Doran Pauline M

机构信息

Westmead Millennium Institute for Medical Research, University of Sydney, Westmead, NSW, 2145, Australia.

Faculty of Science, Engineering and Technology, Swinburne University of Technology, P.O. Box 218, Hawthorn, Melbourne, VIC, 3122, Australia.

出版信息

Methods Mol Biol. 2015;1340:221-33. doi: 10.1007/978-1-4939-2938-2_16.

DOI:10.1007/978-1-4939-2938-2_16

PMID:26445842

Abstract

Mechanical forces, including hydrodynamic shear, hydrostatic pressure, compression, tension, and friction, can have stimulatory effects on cartilage synthesis in tissue engineering systems. Bioreactors capable of exerting forces on cells and tissue constructs within a controlled culture environment are needed to provide appropriate mechanical stimuli. In this chapter, we describe the construction, assembly, and operation of a mechanobioreactor providing simultaneous dynamic shear and compressive loading on developing cartilage tissues to mimic the rolling and squeezing action of articular joints. The device is suitable for studying the effects of mechanical treatment on stem cells and chondrocytes seeded into three-dimensional scaffolds.

摘要

机械力，包括流体动力剪切力、静水压力、压缩力、张力和摩擦力，可对组织工程系统中的软骨合成产生刺激作用。需要能够在可控培养环境中对细胞和组织构建体施加力的生物反应器，以提供适当的机械刺激。在本章中，我们描述了一种机械生物反应器的构建、组装和操作，该反应器能对发育中的软骨组织同时施加动态剪切力和压缩力，以模拟关节的滚动和挤压作用。该装置适用于研究机械处理对接种到三维支架中的干细胞和软骨细胞的影响。