Chang Yu-Han, Wu Min-Hsien
Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Linko, Taiwan.
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan.
Methods Mol Biol. 2015;1340:235-44. doi: 10.1007/978-1-4939-2938-2_17.
In tissue engineering research, cell-based assays are widely utilized to fundamentally explore cellular responses to extracellular conditions. Nevertheless, the simplified cell culture models available at present have several inherent shortcomings and limitations. To tackle the issues, a wide variety of microbioreactors for cell culture have been actively proposed, especially during the past decade. Among these, micro-scale cell culture devices based on microfluidic biochip technology have particularly attracted considerable attention. In this chapter, we not only discuss the advantageous features of using micro-scale cell culture devices for cell-based assays, but also describe their fabrication, experimental setup, and application.
在组织工程研究中，基于细胞的分析方法被广泛用于从根本上探索细胞对细胞外环境的反应。然而，目前可用的简化细胞培养模型存在一些固有的缺点和局限性。为了解决这些问题，人们积极提出了各种各样用于细胞培养的微生物反应器，尤其是在过去十年中。其中，基于微流控生物芯片技术的微型细胞培养装置特别受到了广泛关注。在本章中，我们不仅讨论了使用微型细胞培养装置进行基于细胞的分析的优势特性，还描述了它们的制造、实验设置及应用。
