Department of Mechanical and Industrial Engineering, University of Toronto , 5 King's College Rd., Toronto, Ontario, Canada M5S 3G8.
Urology Research Laboratory, Department of Surgery, Royal Victoria Hospital, McGill University , Montreal, Quebec, Canada H4A 3J1.
J Am Chem Soc. 2015 Nov 4;137(43):13913-9. doi: 10.1021/jacs.5b08523. Epub 2015 Oct 20.
DNA analysis is essential for diagnosis and monitoring of many diseases. Conventional DNA testing is generally limited to the laboratory. Increasing access to relevant technologies can improve patient care and outcomes in both developed and developing regions. Here, we demonstrate direct DNA analysis in paper-based devices, uniquely enabled by ion concentration polarization at the interface of patterned nanoporous membranes in paper (paper-based ICP). Hepatitis B virus DNA targets in human serum are simultaneously preconcentrated, separated, and detected in a single 10 min operation. A limit of detection of 150 copies/mL is achieved without prior viral load amplification, sufficient for early diagnosis of hepatitis B. We clinically assess the DNA integrity of sperm cells in raw human semen samples. The percent DNA fragmentation results from the paper-based ICP devices strongly correlate (R(2) = 0.98) with the sperm chromatin structure assay. In all cases, agreement was 100% with respect to the clinical decision. Paper-based ICP can provide inexpensive and accessible advanced molecular diagnostics.
DNA 分析对于许多疾病的诊断和监测至关重要。传统的 DNA 检测通常局限于实验室。增加对相关技术的获取，可以改善发达国家和发展中国家的患者护理和结果。在这里，我们展示了基于纸张的设备中的直接 DNA 分析，这是通过在纸张中的图案纳米多孔膜界面处的离子浓度极化（基于纸张的 ICP）而独特实现的。在单次 10 分钟的操作中，人血清中的乙型肝炎病毒 DNA 靶标被同时预浓缩、分离和检测。无需事先进行病毒载量扩增即可实现 150 拷贝/mL 的检测限，足以进行乙型肝炎的早期诊断。我们对原始人精液样本中的精子细胞的 DNA 完整性进行临床评估。基于纸张的 ICP 设备的 DNA 碎片化百分比结果与精子染色质结构分析强烈相关（R(2) = 0.98）。在所有情况下，基于临床决策的一致性均为 100%。基于纸张的 ICP 可以提供廉价且易于获取的先进分子诊断。
