在大鼠下丘脑细胞中，减少或增加热休克蛋白72分别会加剧或减轻热诱导的细胞死亡。
Decreasing or increasing heat shock protein 72 exacerbates or attenuates heat-induced cell death, respectively, in rat hypothalamic cells.
作者信息
Lin Kao-Chang, Lin Hung-Jung, Chang Ching-Ping, Lin Mao-Tsun
机构信息
Department of Neurology, Chi Mei Medical Center, Tainan 710, Taiwan ; Department of Biotechnology, Tainan 710, Taiwan.
Department of Biotechnology, Tainan 710, Taiwan ; Department of Emergency Medicine, Chi Mei Medical Center, Tainan 710, Taiwan.
出版信息
FEBS Open Bio. 2015 Sep 3;5:724-30. doi: 10.1016/j.fob.2015.09.001. eCollection 2015.
Abstract
Heat shock protein (HSP) 72 in serum was decreased to a greater degree in patients with serious heat stroke than in those with mild heat stroke. Thus, increased levels of HSP72 appeared to correlate with a better outcome for the patient. Nevertheless, the function of HSP72 in the heat-induced hypothalamic cell death has not been assessed. In this study, we found that increasing HSP72 levels with mild heat preconditioning or decreasing HSP72 levels with pSUPER plasmid expressing HSP72 small interfering RNA significantly attenuated or exacerbated heat-induced cell death in cultured primary hypothalamic cells, respectively. Our findings suggest that HSP72 plays a pivotal role in heat-induced cell death and may be associated with heat tolerance.
摘要
与轻度中暑患者相比，重度中暑患者血清中的热休克蛋白（HSP）72下降幅度更大。因此，HSP72水平升高似乎与患者更好的预后相关。然而，HSP72在热诱导的下丘脑细胞死亡中的作用尚未得到评估。在本研究中，我们发现，用轻度热预处理提高HSP72水平或用表达HSP72小干扰RNA的pSUPER质粒降低HSP72水平，分别可显著减轻或加重培养的原代下丘脑细胞中的热诱导细胞死亡。我们的研究结果表明，HSP72在热诱导的细胞死亡中起关键作用，可能与热耐受性有关。
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