Felgueiras Helena P, Aissa Ines Ben, Evans Margaret D M, Migonney Véronique
Laboratoire de "Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques" (CSPBAT) - UMR CNRS 7244, Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 avenue JB Clément, 93430, Villetaneuse, France.
CSIRO Biomedical Materials Program, 11 Julius Avenue, North Ride, Sydney, NSW, 2113, Australia.
J Mater Sci Mater Med. 2015 Nov;26(11):261. doi: 10.1007/s10856-015-5596-y. Epub 2015 Oct 8.
The research developed on functionalized model or prosthetic surfaces with bioactive polymers has raised the possibility to modulate and/or control the biological in vitro and in vivo responses to synthetic biomaterials. The mechanisms underlying the bioactivity exhibited by sulfonated groups on surfaces involves both selective adsorption and conformational changes of adsorbed proteins. Indeed, surfaces functionalized by grafting poly(sodium styrene sulfonate) [poly(NaSS)] modulate the cellular and bacterial response by inducing specific interactions with fibronectin (Fn). Once implanted, a biomaterial surface is exposed to a milieu of many proteins that compete for the surface which dictates the subsequent biological response. Once understood, this can be controlled by dictating exposure of active binding sites. In this in vitro study, we report the influence of binary mixtures of proteins [albumin (BSA), Fn and collagen type I (Col I)] adsorbed on poly(NaSS) grafted Ti6Al4V on the adhesion and differentiation of MC3T3-E1 osteoblast-like cells and the adhesion and proliferation of Staphylococcus aureus (S. aureus). Outcomes showed that poly(NaSS) stimulated cell spreading, attachment strength, differentiation and mineralization, whatever the nature of protein provided at the interface compared with ungrafted Ti6Al4V (control). While in competition, Fn and Col I were capable of prevailing over BSA. Fn played an important role in the early interactions of the cells with the surface, while Col I was responsible for increased alkaline phosphatase, calcium and phosphate productions associated with differentiation. Poly(NaSS) grafted surfaces decreased the adhesion of S. aureus and the presence of Fn on these chemically altered surfaces increased bacterial resistance ≈70% compared to the ungrafted Ti6Al4V. Overall, our study showed that poly(NaSS) grafted Ti6Al4V selectively adsorbed proteins (particularly Fn) promoting the adhesion and differentiation of osteoblast-like cells while reducing bacterial adhesion to create a bioactive surface with potential for orthopaedic applications.
对具有生物活性聚合物的功能化模型或假体表面开展的研究，提升了调节和/或控制体外及体内对合成生物材料生物学反应的可能性。表面磺化基团所展现出的生物活性背后的机制，涉及吸附蛋白的选择性吸附和构象变化。实际上，通过接枝聚（苯乙烯磺酸钠）[聚（NaSS）]实现功能化的表面，通过诱导与纤连蛋白（Fn）的特定相互作用来调节细胞和细菌反应。生物材料植入后，其表面会暴露于众多竞争该表面的蛋白质环境中，而这决定了后续的生物学反应。一旦了解这一点，就可以通过控制活性结合位点的暴露来加以调控。在这项体外研究中，我们报告了吸附在聚（NaSS）接枝的Ti6Al4V上的蛋白质二元混合物[牛血清白蛋白（BSA）、Fn和I型胶原（Col I）]对MC3T3-E1成骨样细胞的黏附与分化以及金黄色葡萄球菌（金黄色葡萄球菌）的黏附与增殖的影响。结果表明，与未接枝的Ti6Al4V（对照）相比，无论界面处提供何种性质的蛋白质，聚（NaSS）均能刺激细胞铺展、附着强度、分化和矿化。在竞争状态下，Fn和Col I能够胜过BSA。Fn在细胞与表面的早期相互作用中发挥重要作用，而Col I则负责与分化相关的碱性磷酸酶、钙和磷酸盐产量的增加。聚（NaSS）接枝表面降低了金黄色葡萄球菌的黏附，与未接枝的Ti6Al4V相比，这些化学改性表面上Fn的存在使细菌抗性提高了约70%。总体而言，我们的研究表明，聚（NaSS）接枝的Ti6Al4V选择性吸附蛋白质（尤其是Fn），促进成骨样细胞黏附与分化同时减少细菌黏附，从而创建具有骨科应用潜力的生物活性表面。
