Vasconcelos Daniel M, Falentin-Daudré Céline, Blanquaert Daniel, Thomas Damien, Granja Pedro L, Migonney Veronique
INEB - Instituto de Engenharia Biomédica, Universidade do Porto, R. Campo Alegre 823, 4150-180 Porto, Portugal; Faculdade de Engenharia da Universidade do Porto (FEUP), Porto, Portugal; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal.
Laboratory of Biomaterials and Specialty Polymers (LBPS-CSPBAT CNRS UMR 7244), Institut Galilée, Université Paris XIII, Sorbonne Cité, 93430 Villetaneuse, France.
Mater Sci Eng C Mater Biol Appl. 2014 Dec;45:176-83. doi: 10.1016/j.msec.2014.08.054. Epub 2014 Sep 6.
Joint implant-related infections, namely by Staphylococci, are a worldwide problem, whose consequences are dramatic. Various methods are studied to fight against these infections. Here, the proposed solution consists in grafting a bioactive polymer on joint implant surfaces in order to allow the control of the interactions with the living system. In this study, sodium styrene sulfonate, bearing sulfonate groups, was grafted on the surface of titanium alloys. Scanning Electron Microscopy, colorimetric method, Fourier-transformed infrared spectroscopy and contact angle measurements were applied to characterize the surfaces. Bacterial adhesion studies were studied on poly(sodium styrene sulfonate) grafted Ti6Al4V and Ti6Al4V surfaces previously adsorbed by proteins involved in the bacteria adhesion process. Fibrinogen and fibronectin were demonstrated to increase staphylococcal adhesion on Ti6Al4V surfaces. Ti6Al4V grafted sodium styrene sulfonate surfaces inhibited the adhesion of Staphylococcus epidermidis in 37% and 13% on pre-adsorbed surfaces with fibrinogen and fibronectin, respectively. The mechanism of the observed inhibiting bacteria adhesion properties is related to the differences of proteic conformations induced by poly(sodium styrene sulfonate) grafting.
关节植入物相关感染，尤其是由葡萄球菌引起的感染，是一个全球性问题，其后果严重。人们研究了各种方法来对抗这些感染。在此，提出的解决方案是在关节植入物表面接枝一种生物活性聚合物，以便控制与生物系统的相互作用。在本研究中，带有磺酸基团的苯乙烯磺酸钠被接枝到钛合金表面。应用扫描电子显微镜、比色法、傅里叶变换红外光谱和接触角测量来表征表面。对预先被参与细菌粘附过程的蛋白质吸附的聚（苯乙烯磺酸钠）接枝Ti6Al4V和Ti6Al4V表面进行了细菌粘附研究。已证明纤维蛋白原和纤连蛋白会增加葡萄球菌在Ti6Al4V表面的粘附。Ti6Al4V接枝苯乙烯磺酸钠表面在预先吸附有纤维蛋白原和纤连蛋白的表面上分别抑制表皮葡萄球菌粘附37%和13%。观察到的抑制细菌粘附特性的机制与聚（苯乙烯磺酸钠）接枝引起的蛋白质构象差异有关。
