MacCallum Noah, Howell Caitlin, Kim Philseok, Sun Derek, Friedlander Ronn, Ranisau Jonathan, Ahanotu Onye, Lin Jennifer J, Vena Alex, Hatton Benjamin, Wong Tak-Sing, Aizenberg Joanna
Wyss Institute for Biologically Inspired Engineering, ‡School of Engineering and Applied Sciences, and §Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States.
Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, and §Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States.
ACS Biomater Sci Eng. 2015 Jan 12;1(1):43-51. doi: 10.1021/ab5000578. Epub 2014 Dec 24.
There is a dire need for infection prevention strategies that do not require the use of antibiotics, which exacerbate the rise of multi- and pan-drug resistant infectious organisms. An important target in this area is the bacterial attachment and subsequent biofilm formation on medical devices (e.g., catheters). Here we describe nonfouling, lubricant-infused slippery polymers as proof-of-concept medical materials that are based on oil-infused polydimethylsiloxane (iPDMS). Planar and tubular geometry silicone substrates can be infused with nontoxic silicone oil to create a stable, extremely slippery interface that exhibits exceptionally low bacterial adhesion and prevents biofilm formation. Analysis of a flow culture of through untreated PDMS and iPDMS tubing shows at least an order of magnitude reduction of biofilm formation on iPDMS, and almost complete absence of biofilm on iPDMS after a gentle water rinse. The iPDMS materials can be applied as a coating on other polymers or prepared by simply immersing silicone tubing in silicone oil, and are compatible with traditional sterilization methods. As a demonstration, we show the preparation of silicone-coated polyurethane catheters and significant reduction of and biofilm formation on the catheter surface. This work represents an important first step toward a simple and effective means of preventing bacterial adhesion on a wide range of materials used for medical devices.
迫切需要不依赖抗生素的感染预防策略，因为抗生素会加剧多重耐药和泛耐药感染性生物体的增加。这一领域的一个重要目标是防止细菌附着在医疗设备（如导管）上并随后形成生物膜。在此，我们描述了注入润滑剂的非黏附性光滑聚合物，作为基于注入硅油的聚二甲基硅氧烷（iPDMS）的概念验证医用材料。平面和管状几何形状的硅氧烷基底可以注入无毒硅油，以创建一个稳定、极其光滑的界面，该界面具有极低的细菌附着力并可防止生物膜形成。对通过未处理的PDMS和iPDMS管道的流动培养物的分析表明，iPDMS上生物膜形成至少减少了一个数量级，并且在轻轻水洗后，iPDMS上几乎完全没有生物膜。iPDMS材料可以作为涂层应用于其他聚合物上，或者通过简单地将硅氧烷管浸入硅油中来制备，并且与传统灭菌方法兼容。作为演示，我们展示了硅氧烷涂层聚氨酯导管的制备以及导管表面金黄色葡萄球菌和大肠杆菌生物膜形成的显著减少。这项工作代表了朝着一种简单有效的方法迈出的重要第一步，该方法可防止细菌附着在广泛用于医疗设备的材料上。
