Kuśmierska Katarzyna, Szymańska Krystyna, Rokicki Dariusz, Kotulska Katarzyna, Jóźwiak Sergiusz, Sykut-Cegielska Jolanta, Mierzewska Hanna, Szczepanik Elzbieta, Pronicka Ewa, Demkow Urszula
Adv Exp Med Biol. 2016;878:73-82. doi: 10.1007/5584_2015_167.
Metabolites of cerebrospinal biogenic amines (dopamine and serotonin)are an important tool in clinical research and diagnosis of children with neurotransmitter disorders. In this article we focused on finding relationships between the concentration of biogenic amine metabolites, age, and gender. We analyzed 148 samples from children with drug resistant seizures of unknown etiology and children with mild stable encephalopathy aged 0-18 years. A normal profile of biogenic amineswas found in 107 children and those children were enrolled to the study group. The CSF samples were analyzed by HPLC with an electrochemical detector. The concentrations of the dopamine and serotonin metabolites homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), respectively, were high at birth, gradually decreasing afterward until the 18 years of age. Nevertheless, the HVA/5-HIAA ratio did not vary with age, except in the children below 1 year of age. In the youngest group we observed a strong relationship between the HVA/5-HIAA ratio and age (r = 0.69, p < 0.001). There were no statistical differences in the level of both dopamine and serotonin metabolites between boys and girls, although a tread toward lower HVA and 5-HIAA in the boys was noticeable. Significant inter-gender differences in the level of HVA and 5-HIAA were noted only in the age-group of 1-4 years, with 5-HIAA being higher in the girls than boys (p = 0.004). In conclusion, the study revealed that the concentration of biogenic amine metabolites is age and sex dependent.
脑脊液生物胺（多巴胺和血清素）的代谢产物是临床研究和诊断神经递质紊乱儿童的重要工具。在本文中，我们着重研究生物胺代谢产物浓度、年龄和性别的关系。我们分析了148份样本，这些样本来自病因不明的耐药性癫痫儿童以及0至18岁患有轻度稳定型脑病的儿童。在107名儿童中发现了生物胺的正常谱型，这些儿童被纳入研究组。脑脊液样本采用带电化学检测器的高效液相色谱法进行分析。多巴胺和血清素的代谢产物高香草酸（HVA）和5-羟吲哚乙酸（5-HIAA）的浓度在出生时较高，之后逐渐下降，直至18岁。然而，HVA/5-HIAA比值除1岁以下儿童外，并不随年龄变化。在最年幼的组中，我们观察到HVA/5-HIAA比值与年龄之间存在很强的相关性（r = 0.69，p < 0.001）。男孩和女孩的多巴胺和血清素代谢产物水平无统计学差异，尽管男孩的HVA和5-HIAA有降低趋势。仅在1至4岁年龄组中发现HVA和5-HIAA水平存在显著的性别差异，女孩的5-HIAA高于男孩（p = 0.004）。总之，该研究表明生物胺代谢产物的浓度与年龄和性别有关。
