Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK.
Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UK.
Stem Cell Reports. 2015 Nov 10;5(5):690-701. doi: 10.1016/j.stemcr.2015.09.003. Epub 2015 Oct 8.
Despite advances in our understanding of interactions between mouse hematopoietic stem cells (HSCs) and their niche, little is known about communication between human HSCs and the microenvironment. Using a xenotransplantation model and intravital imaging, we demonstrate that human HSCs display distinct motile behaviors to their hematopoietic progenitor cell (HPC) counterparts, and the same pattern can be found between mouse HSCs and HPCs. HSCs become significantly less motile after transplantation, while progenitor cells remain motile. We show that human HSCs take longer to find their niche than previously expected and suggest that the niche be defined as the position where HSCs stop moving. Intravital imaging is the only technique to determine where in the bone marrow stem cells stop moving, and future analyses should focus on the environment surrounding the HSC at this point.
尽管我们对小鼠造血干细胞 (HSC) 与其龛位之间的相互作用有了更深入的了解，但对于人类 HSC 与微环境之间的通信却知之甚少。通过异种移植模型和活体成像，我们证明了人类 HSC 表现出与其造血祖细胞 (HPC) 不同的迁移行为，而在小鼠 HSC 和 HPC 之间也可以发现相同的模式。HSC 在移植后明显变得不那么活跃，而祖细胞仍然活跃。我们发现人类 HSC 找到其龛位所需的时间比之前预期的要长，并且表明龛位应定义为 HSC 停止移动的位置。活体成像技术是唯一可以确定骨髓干细胞在何处停止移动的技术，未来的分析应该集中在该位置的 HSC 周围的环境上。
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